摘要
Microfluidic phenotyping methods have been of vital importance for cellular characterization,especially for evaluating single cells.In order to study the deformability of a single cell,we devised and tested a tunable microfluidic chip-based method.A pneumatic polymer polydimethylsiloxane(PDMS)membrane was designed and fabricated abutting a single-cell trapping structure,so the cell could be squeezed controllably in a lateral direction.Cell contour changes under increasing pressure were recorded,enabling the deformation degree of different types of single cell to be analyzed and compared using computer vision.This provides a new perspective for studying mechanical properties of cells at the single cell level.
基金
The authors gratefully acknowledge financial support from National Key R&D Program of China(2018YFE0118700)
the National Natural Science Foundation of China(NSFC No.62174119)
the 111 Project(B07014)
the Foundation for Talent Scientists of Nanchang Institute for Micro-technology of Tianjin University.
