HPLC-QAMS法同时测定何首乌防脱育发精华液中9种成分含量

Simultaneous determination of nine effective components in Polygonum Multif lorum Fangtuoyufa Jinghuaye by HPLC-QAMS

建立高效液相色谱一测多评法(HPLC-QAMS法)同时检测何首乌防脱育发精华液中9种成分的含量,并验证所建立方法的可行性和准确性。以槲皮苷为含量参照物,甲醇-0.1%磷酸为流动相,分别采用外标法和HPLC-QAMS法同时测定12批何首乌防脱育发精华液中大黄素、大黄素甲醚、杨梅苷、槲皮苷、杨梅素、槲皮素、山柰酚、穗花杉双黄酮、扁柏双黄酮的含量,对比两种方法的结果差异,验证HPLC-QAMS法的适用性。结果显示,9种成分各线性范围良好,相关系数均大于0.999,检出限为0.027~0.151μg/mL,定量限为0.076~0.449μg/mL。以槲皮苷为内参比物质,大黄素、大黄素甲醚、杨梅苷、杨梅素、槲皮素、山柰酚、穗花杉双黄酮、扁柏双黄酮的相对校正因子分别为0.7537,0.8369,1.0192,2.9913,1.6875,2.2369,1.0963和1.2493。两种方法测得的何首乌防脱育发精华液中9种成分的含量差异均无统计学意义(P>0.05)。以槲皮苷为含量参照物建立的相对校正因子重现性好,HPLC-QAMS法可用于何首乌防脱育发精华液中9种成分的质量评价。

The study aimed to establish an HPLC-QAMS method for simultaneous quantitation of nine effective components in Polygonum Multiflorum Fangtuoyufa Jinghuaye,and to verify the feasibility and accuracy of the method.Quercitrin was used as the reference substance and methanol-0.1%phosphoric acid was used as the mobile phase.The contents of emodin,physcion,myricitrin,quercitrin,myricetin,quercetin,kaempferol,amentoflavone and hinokiflavone in 12 batches of Polygonum Multiflorum Fangtuoyufa Jinghuaye were determined by external standard method and HPLC-QAMS method,respectively,and the difference between the results of the two methods was compared to verify the serviceability of HPLC-QAMS method.The results show that the linear ranges of the nine components are good,the correlation coefficients are greater than 0.999,the limits of detection(LOD)are 0.027-0.151μg/mL,and the limits of quantification are 0.076-0.449μg/mL.The relative correlation factors are 0.7537,0.8369,1.0192,2.9913,1.6875,2.2369,1.0963 and 1.2493 for emodin,physcion,myricitrin,myricetin,quercetin,kaempferol,amentoflavone and hinokiflayone,respectively.There is no significant difference in the content of 9 components in Polygonum Multiflorum Fangtuoyufa Jinghuaye determined by the two methods(P>0.05).The relative correlation factors established with quercitrin as the internal reference substance have good reproducibility.The HPLC-QAMS method can be used for the quality evaluation of nine effective components in Polygonum Multiflorum Fangtuoyufa Jinghuaye.