冲击波对糖尿病大鼠股动脉血管黏附分子及血流动力学的影响

Effects of shock wave on vascular adhesion molecules and hemodynamics of femoral artery in diabetic rats

目的:探讨冲击波对糖尿病大鼠股动脉血管黏附分子及血流动力学的影响。方法:清洁级健康雄性SD大鼠18只,10周龄,体重220g—250g,随机分为3组(n=6):对照组(A组)、糖尿病组(B组)、冲击波治疗组(C组)。B组和C组通过高糖高脂饮食及腹腔注射链脲菌素建立糖尿病模型,C组在模型建立成功后第1、2、3、4周时各接受1次冲击波治疗。治疗结束后,超声观察各组大鼠股动脉直径和多普勒血流变化,并取各组左侧股动脉,蛋白质印迹法(Western Blot)检测ICAM-1、VCAM-1蛋白表达及反转录聚合酶链式反应(RTPCR)检测ICAM-1 mRNA、VCAM-1 m RNA的表达。结果:与A组比较,B组、C组大鼠ICAM-1、VCAM-1蛋白表达及ICAM-1 mRNA、VCAM-1 mRNA表达升高(P<0.05);与B组比较,C组ICAM-1、VCAM-1蛋白表达及ICAM-1 m RNA、VCAM-1 mRNA表达降低(P<0.05);与A组比较,B、C组大鼠股动脉直径及血流量明显减小(P<0.05);与B组比较,C组大鼠股动脉直径及血流量增大(P<0.05)。结论:冲击波治疗可以改善糖尿病大鼠股动脉直径,增加股动脉血流量,可能与减少ICAM-1、VCAM-1蛋白表达、ICAM-1 mRNA、VCAM-1 mRNA的表达有关。

Objective:To investigate the effects of shock wave on femoral artery adhesion molecules and hemodynamics in diabetic rats.Method:Eighteen healthy male SD rats aged 10 weeks with a body weight of 220g—250g were randomly divided into 3 groups:control group(group A,n=6),diabetes group(group B,n=6)and shock wave treatment group(group C,n=6).Group B and C were treated with high glucose and high fat diet and intraperitoneal injection of streptozotocin to establish diabetic models.Group C was treated with shock wave therapy at 1,2,3 and 4 weeks after successful model establishment.After the treatment,the changes in femoral artery diameter and Doppler blood flow in each group were observed by ultrasonography.The left femoral artery of each group was taken to detect the protein expressions of ICAM-1 and VCAM-1 by Western Blot and the mRNA expressions of ICAM-1 and VCAM-1 by RT-PCR.Result:Compared with group A,the protein expression of ICAM-1 and VCAM-1 and mRNA expression of ICAM-1 and VCAM-1 in group B and C were increased(P<0.05);compared with group B,the protein expression of ICAM-1 and VCAM-1 and mRNA expression of ICAM-1 and VCAM-1 in group C were de-creased(P<0.05);compared with group A,the diameter and blood flow of femoral artery in groups B and C were significantly decreased(P<0.05);compared with group B,the diameter and blood flow of femoral artery in group C increased(P<0.05).Conclusion:Shock wave therapy can improve the diameter of femoral artery and increase the blood flow of femoral artery in diabetic rats.It may be related to the decrease of the expression of ICAM-1,VCAM-1 protein,ICAM-1 mRNA and VCAM-1 mRNA.