老年结直肠癌肝转移病人IL-18、PRL-3表达与PI3K/AKT信号通路关系探究

Relationship between IL-18,PRL-3 and PI3K/AKT signaling pathways in elderly patients with colorectal cancer complicated with liver metastases

目的 探讨老年结直肠癌(CRC)肝转移病人IL-18、促肝再生磷酸酶蛋白(PRL-3)的表达水平与磷脂酰肌醇-3-激酶(PI3K)/蛋白激酶B(AKT)信号通路关系。方法 选取我院2019年6月至2021年6月100例老年CRC病人,其中肝转移病人34例,非肝转移病人66例。采用蛋白免疫印迹法检测病人癌组织及癌旁组织中IL-18、PRL-3、PI3K、AKT蛋白表达情况并进行比较分析。采用Logistic回归分析影响老年CRC肝转移的危险因素,Pearson相关系数评估肝转移病人IL-18、PRL-3表达与PI3K/AKT信号通路的关系。结果 癌组织中IL-18、PRL-3、PI3K及AKT蛋白表达水平均高于癌旁组织(P<0.05);肝转移病人癌组织中IL-18、PRL-3、PI3K及AKT蛋白表达水平均高于非肝转移病人(P<0.05);肝转移病人癌灶浸润深度T3~T4、低中分化、淋巴结转移比例均高于非肝转移病人(P<0.05);Logistic回归分析显示,IL-18、PRL-3、PI3K及AKT蛋白阳性表达、癌灶浸润深度T3~T4、低中分化、淋巴结转移均为老年CRC肝转移的独立危险因素(P<0.05);Pearson相关分析显示,老年CRC肝转移病人癌组织中IL-18、PRL-3蛋白表达水平与PI3K、AKT蛋白表达水平呈正相关(P<0.05)。结论 老年CRC肝转移病人IL-18、PRL-3呈高表达状态,两者表达水平均与PI3K、AKT蛋白水平呈正相关,IL-18、PRL-3是老年CRC肝转移的独立危险因素。

Objective To explore the relationship of interleukin-18(IL-18) and phosphatase of regenerating liver-3(PRL-3) with phosphatidylinositol-3-kinase(PI3K)/protein kinase B(AKT) signaling pathways in the elderly patients with colorectal cancer(CRC) complicated with liver metastases.Methods A total of 100 elderly patients with CRC in our hospital were enrolled from June 2019 to June 2021.The expressions of IL-18,PRL-3,PI3K and AKT proteins in cancer tissues and para-carcinoma tissues were detected by Western Blot and compared.According to presence or absence of liver metastases,all patients were divided into metastases group(n=34) and non-metastases group(n=66).The risk factors of liver metastases were analyzed by Logistic regression analysis.The relationship between IL-18,PRL-3 and PI3K/AKT signaling pathways in liver metastases patients was analyzed by Pearson correlation.Results The expression levels of IL-18,PRL-3,PI3K and AKT proteins in cancer tissues were higher than those in para-carcinoma tissues(P<0.05),which were also higher in metastases group than those in non-metastases group(P<0.05).The proportions of cases with tumor invasion depth of T3-T4,low and moderate differentiation and lymph node metastasis in metastases group were higher than those in non-metastases group(P<0.05).Logistic regression analysis showed that the positive expressions of IL-18,PRL-3,PI3K and AKT proteins,tumor invasion depth of T3-T4,low and moderate differentiation,and lymph node metastasis were all independent risk factors of liver metastases in the elderly patients with CRC(P<0.05).Pearson correlation analysis showed that the expressions of IL-18 and PRL-3 proteins were positively correlated with the expressions of PI3K and AKT proteins in liver metastases patients(P<0.05).Conclusions The expressions of IL-18 and PRL-3 are up-regulated in the elderly patients with CRC and liver metastases,which are positively correlated with PI3K and AKT proteins.IL-18 and PRL-3 are independent risk factors of liver metastases in the elderly patients with CRC.