Notch3、LGR5及NF-κB在大肠腺癌组织中的表达及意义

Expression and clinical significance of Notch3,LGR5 and NF-κB in colorectal adenocarcinoma

目的 探讨大肠腺癌中Notch3、富含亮氨酸重复序列G蛋白偶联受体5(LGR5)及核转录因子κB(NF-κB)的表达及其临床意义。方法 选取2013年1月—2015年10月遵义医科大学附属医院大肠腺癌标本(腺癌组)54例、腺瘤(腺瘤组)30例、正常大肠黏膜组织(正常组)12例,采用免疫组化法,对各组织中的Notch3,LGR5,NF-κB的表达进行检测。结果 在正常大肠黏膜组、腺瘤组及腺癌组中,Notch3、LGR5、NF-κB的阳性率均呈逐渐递增趋势,Notch3、LGR5和NF-κB在大肠腺癌组织中的阳性率分别为63%(34/54)、57.4%(31/54)、63%(34/54),分别显著高于其在正常组的阳性率(8.3%、16.7%、25%)(P<0.05)和腺瘤组的阳性率(26.7%、36.7%、30%)。腺瘤组的阳性率与正常组相比,差异无统计学意义(P>0.05)。上述指标的表达与大肠癌患者年龄、性别、肿瘤发生部位、肿瘤分化程度及肿瘤直径等均不相关(P>0.05);但均与淋巴结转移和Dukes分期有关(P<0.05)。Notch3及NF-κB的表达还与肿瘤浸润深度有关(P<0.05);Notch3在大肠腺癌中的表达与LGR5呈正相关(r=0.325,P<0.05)。结论 Notch3、LGR5、NF-κB可能参与了大肠腺癌的发生、发展过程,有望成为新的肿瘤标志物,作为大肠癌的早期诊断及预后评价指标。

Objective To investigate the expression and clinical significance of Notch3,LGR5and NF-κB in colorectal adenocarcinoma.Methods The expression of Notch3,LGR5 and NF-κB in 54 colorectal adenocarcinoma tissue,30 colorectal adenoma tissue and 12 normal colorectal mucosa tissue were detected by immunohistochemistry.Results The positive rates of Notch3,LGR5 and NF-κB in normal colorectal tissue group,adenoma group and adenocarcinoma group were gradually increasing.Their positive expression rates in colorectal adenocarcinoma group were 63%(34/54),57.4%(31/54)and 63%(34/54),respectively,significantly higher than that of normal group(8.3%,16.7%,25%)(P<0.05)and adenoma group(26.7%,36.7%,30%).There was no significant difference between the adenoma group and the normal group(P>0.05).The above indicators weren't correlated with age,gender,tumor site,degree of differentiation and the diameter of tumor(P>0.05).The expression of Notch3,LGR5 and NF-κB in colorectal adenocarcinoma were all related to lymph node metastasis and Dukes stage(P<0.05).Notch3 and NF-κB were also related to the tumor infiltration depth(P<0.05).Notch3 was positively correlated with LGR5's expression in colorectal adenocarcinoma(r=0.325,P<0.05).Conclusion Notch3,LGR5 and NF-κB may be involved in the occurrence and development of colorectal adenocarcinoma.