乳腺癌组织中E-cadherin和Vimentin表达水平与新辅助化疗疗效分析

Analysis of the expression of E-cadherin and Vimentin in breast cancer tissues and the efficacy of neoadjuvant chemotherapy

目的 对乳腺癌穿刺组织标本进行免疫组化分析,收集E-Cadherin和Vimentin的表达水平,探讨两者的表达水平与新辅助化疗疗效是否具有相关性。方法 收集2019—2021年在本院甲乳病区治疗的新辅助化疗乳腺癌患者作为研究对象,所有患者术前完成新辅助化疗疗程,且成功实施手术,通过免疫组化的方法检测术前穿刺标本中E-Cadherin和Vimentin的表达情况,术后对新辅助化疗疗效进行MP分级。结果 最终筛选出符合入选标准并接受全程治疗的患者共96例,均为女性患者,其中E-Cadherin高表达42例,低表达54例;Vimentin高表达60例,低表达36例。E-Cadherin和Vimentin的表达水平与初治时的相关病理资料密切相关(P<0.05),包括肿瘤的大小、HER-2表达、ki-67水平以及腋窝淋巴结有没有出现转移。对新辅助化疗疗效进行分析显示,高表达的E-Cadherin且低表达的Vimentin的组别化疗疗效高于其他组,差异有统计学意义(P<0.05)。进一步对不同分子分型的患者进行分析,显示HER-2阳性型的疗效好于三阴型,好于Luminal型,且差异有统计学意义(P<0.05)。结论 新辅助化疗在E-Cadherin高表达且vimentin低表达以及分子型HER-2阳性型的人群中,有着更好的效果。因此在新辅助化疗治疗前检测乳腺癌穿刺标本中E-Cadherin和vimentin的表达情况,能够成为NAC疗效的预测因子。

Objective To collect the expression levels of E-cadherin and Vimentin by immunohistochemical analysis of breast cancer puncture tissue samples,and to explore whether the expression levels of E-cadherin and Vimentin are related to the efficacy of neoadjuvant chemotherapy.Methods Patients suffered from breast cancer those were treated with neoadjuvant chemotherapy in the thyroid gland and breast ward of the first affiliated hospital of Bengbu Medical College were enrolled as study subjects.All patients completed the course of neoadjuvant chemotherapy before surgery and successfully performed surgery.Detect the expression of E-cadherin and Vimentin in preoperative puncture samples by immunohistochemical method,and the efficacy of neoadjuvant chemotherapy was gradedby MP method after surgery.Results A total of 96 patients who met the inclusion criteria and received full course treatment were finally selected,all of them were female patients,including 42 cases of high expression of E-Cadherin,54 cases of low expression,60 cases of high expression of Vimentin,and 36 cases of low expression.The expression levels of E-cadherin and Vimentin were closely related to the relevant pathological data at the time of initial treatment(P<0.05),including tumor size,HER-2 expression,ki-67 level and whether exist metastasis in axillary lymph nodes.The analysis of the efficacy of neoadjuvant chemotherapy showed that the chemotherapy efficacy of the group with high expression of E-cadherin and low expression of Vimentin was higher than that of other groups,and the difference was statistically significant(P<0.05).Further analysis of patients with different molecular types showed that the curative effect of HER-2 positive type was better than that of three negative type and Luminal type,and the differences were statistically significant(P<0.05).Conclusions Neoadjuvant chemotherapy has a better effect in the population of high expression of E-Cadherin and low expression of vimentin,as well as in the population of HER-2 positive type.Therefore,detecting the expression of E-Cadherin and vimentin in breast cancer puncture specimens before neoadjuvant chemotherapy can become a predictor of NAC efficacy.