日粮中性洗涤纤维水平对犊牛肠道抗氧化能力的影响

Effects of Dietary Neutral Detergent Fiber Level on Intestinal Antioxidant Capacity of Calves

为明确中性洗涤纤维(NDF)水平对犊牛肠道组织抗氧化应激能力的影响,试验选用24头初生重相近[(42±2.5)kg]的新生荷斯坦公犊牛,随机分为4组,分别饲喂NDF水平为10%(10%NDF组)、15%(15%NDF组)、20%(20%NDF组)和25%(25%NDF组)的日粮。结果表明:空肠抗氧化指标:总抗氧化能力(T-AOC)10%NDF组显著高于20%NDF组(P<0.05),15%NDF组极显著高于20%NDF组和25%NDF组(P<0.01);丙二醛(MDA)含量25%NDF组显著高于10%NDF组(P<0.05)。回肠和十二指肠抗氧化指标各组均无显著性差异(P>0.05)。空肠mRNA表达水平:谷胱甘肽过氧化物酶(GSH-Px)的mRNA相对表达量10%NDF组显著高于15%NDF组(P<0.05),25%NDF组极显著高于15%NDF组(P<0.01)、显著高于20%NDF组(P<0.05);超氧化物歧化酶1(SOD1)的mRNA相对表达量25%NDF组显著高于15%NDF组(P<0.05);谷胱甘肽S转移酶A1(GSTA1)的mRNA相对表达量20%NDF组极显著高于10%NDF组、15%NDF组(P<0.01),25%NDF组显著高于10%NDF组(P<0.05)。回肠mRNA表达水平:GSH-Px的mRNA相对表达量15%NDF组极显著高于10%NDF组、25%NDF组(P<0.01)、显著高于20%NDF组(P<0.05),20%NDF组显著高于25%NDF组(P<0.05);SOD1的mRNA相对表达量25%NDF组极显著低于其余3组(P<0.01);CAT的mRNA相对表达量15%NDF组极显著高于其余3组(P<0.01),10%NDF组和20%NDF组极显著高于25%NDF组(P<0.01);GSTA1的mRNA相对表达量15%NDF组极显著高于其余3组(P<0.01),10%NDF组显著高于25%NDF组(P<0.05)。十二指肠mRNA表达水平:GSH-Px的mRNA相对表达量15%NDF组极显著低于其余3组(P<0.01),10%NDF组显著高于20%NDF组、25%NDF组(P<0.05);SOD1的mRNA相对表达量25%NDF组极显著高于其余3组(P<0.01);CAT的mRNA相对表达量15%NDF组极显著高于其余三组(P<0.01)。试验表明:日粮中15%和20%水平的NDF可以提高犊牛肠道的抗氧化能力。

To determine the effect of neutral detergent fiber(NDF)level on the antioxidant stress capacity of intestinal tissue in calves,24 newborn Holstein male calves with a similar birth weight[(42±2.5)kg]were randomly divided into four groups.Diets with NDF levels of 10%(10%NDF group),15%(15%NDF group),20%(20%NDF group),and 25%(25%NDF group)were fed.The results showed that the total antioxidant capacity(T-AOC)of jejunum in 10%NDF group was significantly higher than that in 20%NDF group(P<0.05),and that in 15%NDF group was significantly higher than that in 20%NDF group and 25%NDF group(P<0.01).The content of malondialdehyde(MDA)in 25%NDF group was significantly higher than that in 10%NDF group(P<0.05).There were no significant differences in the antioxidant indexes of ileum and duodenum among all groups(P>0.05).The relative expression of GSH-Px mRNA in jejunum in 10%NDF group was significantly higher than that in 15%NDF group(P<0.05),and the relative expression of GSH-Px mRNA in 25%NDF group was significantly higher than that in 15%NDF group(P<0.01)and significantly higher than that in 20%NDF group(P<0.05).The relative expression of superoxide dismutase one(SOD1)mRNA in the 25%NDF group was significantly higher than that in the 15%NDF group(P<0.05).The relative expression of glutathione S-transferase Aone(GSTA1)mRNA in 20%NDF group was significantly higher than that in 10%NDF group and 15%NDF group(P<0.01),and that in 25%NDF group was significantly higher than that in 10%NDF group(P<0.05).The relative expression level of GSH-Px mRNA in ileum of 15%NDF group was significantly higher than that of 10%NDF group and 25%NDF group(P<0.01),significantly higher than that of 20%NDF group(P<0.05),and 20%NDF group was significantly higher than 25%NDF group(P<0.05).The relative expression of SOD1 mRNA in 25%NDF group was significantly lower than that in other three groups(P<0.01).The relative expression of CAT mRNA in 15%NDF group was significantly higher than that in other three groups(P<0.01),that in 10%NDF group and 20%NDF group was significantly higher than that in 25%NDF group(P<0.01).The relative expression of GSTA1 mRNA in 15%NDF group was significantly higher than that in other three groups(P<0.01),and that in 10%NDF group was significantly higher than that in 25%NDF group(P<0.05).The mRNA expression of GSH-Px in duodenum of 15%NDF group was significantly lower than that of the other three groups(P<0.01),and the mRNA expression of 10%NDF group was significantly higher than that of 20%NDF group and 25%NDF group(P<0.05).The relative expression of SOD1 mRNA in 25%NDF group was significantly higher than that in other three groups(P<0.01).The relative expression of CAT mRNA in 15%NDF group was significantly higher than that in other three groups(P<0.01).The results showed that dietary NDF at 15%and 20%levels could improve the antioxidant capacity of the calf intestine.