XapR蛋白在嗜水气单胞菌抗生素耐药性中的功能

The Function of XapR Protein in Antibiotic Resistance of Aeromonas Hydrophila

研究嗜水气单胞菌(Aeromonas hydrophila)LysR型转录调节因子中XapR蛋白抗生素耐药的调控机制。通过测定嗜水气单胞菌ATCC 7966(野生型菌株)与嗜水气单胞菌ATCC 7966 xapR基因缺失株(ΔxapR)对32种抗生素的最低抑菌浓度,系统地评价xapR基因抗生素耐药性;进一步利用定量蛋白质组学技术,探讨xapR参与嗜水气单胞菌调控的生物过程。结果表明:xapR基因缺失后细菌对头孢孟多的耐药性减弱;xapR基因可调控细菌碳代谢、TCA循环等多种代谢途径,此外还与外膜蛋白、TonB、ABC转运系统等抗性基因的表达密切相关。综上,XapR蛋白可通过调节细菌中心代谢途径以及多个抗性基因改变嗜水气单胞菌的耐药性。

This paper studied the regulation mechanism on antibiotic resistance of LTTRs family protein XapR in Aeromo⁃nas hydrophila.The Minimum bactericidal concentration of Aeromonas hydrophila ATCC 7966(wild-type strain)and Aeromonas hydrophila ATCC 7966 xapR gene deletion strain(ΔxapR)against 32 antibiotics were measured to evaluate the antibiotic resistance.Further,quantitative proteomics were used to investigate the effect of xapR deletion on the pro⁃tein expression of Aeromonas hydrophila.The results showed that by contrast with wild-type strains in terms of antibiotics resistance,the minimum bactericidal concentration of cefamandole reduced by 2 times inΔxapR.Subsequently,omics analysis showed that xapR could regulate bacterial carbon metabolism,TCA cycle and other pathways,and also closely related to the expressions of outer membrane protein,TonB,and ABC transport system.In conclusion,XapR can change the drug resistance of Aeromonas hydrophila by regulating the central metabolic pathway and multiple resistance genes.