淫羊藿药材中黄酮类成分提取工艺优选

Optimization of Extraction Process for Flavonoids in Epimedii Folium

目的优选淫羊藿药材中黄酮类成分提取工艺。方法采用高效液相色谱法测定淫羊藿药材中朝藿定A1、朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷Ⅰ含量。在单因素试验基础上,以提取溶剂、液料比、提取时间、提取次数为考察因素,待测成分提取总量为考察指标,采用响应面法优选淫羊藿药材中黄酮类成分的提取工艺。结果最佳提取工艺为液料比100(mL/g)时,以50%乙醇提取2次,每次40 min。验证试验结果显示,待测成分提取总量分别为3.16%,3.08%,3.03%,与预测值(3.12%)相当。结论该提取工艺合理可行,可为淫羊藿药材的进一步研发提供参考。

Objective To optimize the extraction process of flavonoids in Epimedii Folium.Methods The contents of epimedin A1,epimedin A,epimedin B,epimedin C,icariin and baohuosideⅠin Epimedii Folium were determined by the high-performance liquid chromatography(HPLC)method.Based on the single factor test,the extraction process of flavonoids in Epimedii Folium was optimized by the response surface methodology,with the extraction solvent,liquid-solid ratio,extraction time and extraction frequency as the investigation factors,and with the total extraction amount of components to be tested as the investigation indicator.Results The optimal extraction process was as follows:the liquid-solid ratio was 100(mL/g),and extracting twice with 50%ethanol,each time for 40 min.The verification test showed that the total extraction amounts of components to be tested were 3.16%,3.08%,3.03%,respectively,which were similar to the predicted value(3.12%).Conclusion The extraction process is reasonable and feasible,which can provide a reference for the further development of Epimedii Folium.