补锌对高脂饮食大鼠睾丸组织circRNA-miRNA-mRNA网络的影响

Effects of zinc supplementation for rats fed with high fat diet on their circRNA,microRNA,and mRNA network in thetesticular tissue

目的:探讨补锌对高脂饮食雄性大鼠睾丸组织环状RNA(circRNA)和微小RNA(miRNA)表达谱的影响。方法:通过构建高脂饮食和补锌大鼠模型,取两组大鼠睾丸组织进行高通量RNA测序,筛选差异表达的circRNAs和miRNAs,并进行GO和KEGG功能富集分析,随后构建circRNA-miRNA-mRNA网络。结果:筛选了54个差异表达circRNAs、2个差异表达miRNAs;GO分析结果显示脂肪酸氧化、脂蛋白代谢和生物合成过程、肽转运ATP酶活性和氧化还原酶活性等过程发挥作用;KEGG富集分析显示富集在TNF信号通路、cAMP信号通路和AMPK信号通路等通路;筛选出差异显著且具有靶向关系的circRNA和miRNA:novel_circ_0016964、novel_circ_0013783和rno-miR-540-3p,随后预测rno-miR-540-3p的靶基因,并构建circRNA-miRNA-mRNA ceRNA网络。结论:补锌使高脂饮食大鼠生殖损伤过程中circRNA和miRNA表达谱发生变化,故circRNA和miRNA可能是其发挥作用的潜在靶点。

Objective:To investigate the effects of zinc supplementation for male rats fed with high fat diet on their circRNA and microRNA(miRNA)expression profiles in the testicular tissue.Methods:The rat models with high-fat diet and zinc supplementation were established in two groups,and the testicular tissues of the rats in the two groups were selected for the high-throughput RNA sequencing,and the differentially expressions of circRNAs and miRNAs were screened.GO and KEGG functional enrichment analysis was performed,and then the circRNA,-miRNA,andmRNA network was constructed.Results:There were 54differentially expressed circRNAs screened and 2differentially expressed miRNAs screened.The results of GO analysis showed that the fatty acid oxidation,the lipoprotein metabolism and biosynthesis,the peptide transport ATPase activity,and the oxidoreductase activity played their roles.KEGG enrichment analysis showed that the signaling pathways of the TNF,the cAMP,and the AMPK had been enriched.The circRNA and miRNA with significant differences and with targeting relationship were screened,which including novel_circ_0016964,novel_circ_0013783,and rno-miR-540-3p,and then the target gene of rno-miR-540-3p was predicted,and the ceRNA network of circRNA,miRNA,and mRNA was constructed.Conclusion:Zinc supplementation for the rats fed with high-fat diet can change the expression profiles of their circRNA and miRNA during their reproductive injury,so circRNA and miRNA may be as the potential targets for playing their roles.