三阴性乳腺癌组织中ZNF545表达与预后及腋窝淋巴结转移的关系

Relationship between ZNF545 expression and prognosis and axillary lymph node metastasis in triple negative breast cancer

目的:探讨三阴性乳腺癌组织锌指蛋白545(ZNF545)的表达与预后及腋窝淋巴结转移的关系。方法:采用免疫组织化学染色法检测107例三阴性乳腺癌患者癌组织中ZNF545、Wnt/β-catenin信号通路相关蛋白(β-catenin和C-myc)表达水平。采用Logistic多因素分析影响三阴性乳腺癌患者腋窝淋巴结转移的影响因素。Kaplan-Meier法用于三阴性乳腺癌患者预后的单因素分析,Cox比例风险回归模型用于三阴性乳腺癌患者预后的多因素分析。结果:三阴性乳腺癌组织中ZNF545低表达者50例(46.7%)。ZNF545低表达患者腋窝淋巴结转移高于ZNF545高表达患者(χ^(2)=4.709,P=0.030),ZNF545表达水平与β-catenin、C-myc表达水平均呈负相关(r值分别为-0.553和-0.551,均P<0.001)。ZNF545低表达患者的5年总生存(OS)率(58.0%)低于ZNF545高表达患者(84.2%),差异有统计学意义(χ^(2)=9.839,P=0.002)。多因素分析显示,ZNF545低表达(OR=2.462,95%CI 1.186~5.750,P=0.017)、组织学分级低(OR=2.650,95%CI 1.101~4.924,P=0.027)及有淋巴结转移(OR=6.307,95%CI 2.651~16.351,P<0.001)是三阴性乳腺癌患者预后的独立危险因素。ZNF545低表达是三阴性乳腺癌患者腋窝淋巴结转移的独立危险因素(OR=2.355,95%CI 1.080~5.134,P=0.031),且ZNF545表达水平与腋窝淋巴结转移数目呈负相关(r=-0.3241,P=0.013)。结论:ZNF545低表达是三阴性乳腺癌患者预后和腋窝淋巴结转移的独立危险因素,其可能通过影响Wnt/β-catenin信号通路相关蛋白参与三阴性乳腺癌的进展。

Objective:To investigate the relationship between zinc finger protein 545(ZNF545)expression and prognosis and axillary lymph node metastasis in triple negative breast cancer(TNBC).Methods:The expression levels of ZNF545 and Wnt/β-catenin signaling pathway related proteins(β-catenin and C-myc)in 107 cancer issues of TNBC patients were detected by immunohistochemical staining.Logistic multivariate analysis was used to analyze the influencing factors of axillary lymph node metastasis of TNBC.Kaplan-Meier method was used to analyze the single factor survival of patients with TNBC.Cox proportional hazard regression model was used to analyze the independent prognostic factors of TNBC patients.Results:There were 50 cases(46.7%)with low expression of ZNF545 in TNBC.The axillary lymph node metastasis rate of ZNF545 low expression group was higher than that of ZNF545 high expression group(χ^(2)=4.709,P=0.030).The expression level of ZNF545 was negatively correlated with the expression levels ofβ-catenin and C-myc(r=-0.553 and-0.551,respectively,P<0.001).The 5-year overall survival(OS)rate of ZNF545 low expression group(58.0%)was lower than that of ZNF545 high expression group(84.2%),and the difference was statistically significant(χ^(2)=9.839,P=0.002).Multivariate analysis showed that lower expression of ZNF545(OR=2.462,95%CI 1.186-5.750,P=0.017),lower histological grade(OR=2.650,95%CI 1.101-4.924,P=0.027)and lymph node metastasis(OR=6.307,95%CI 2.651-16.351,P<0.001)were independent prognostic risk factors for patients with TNBC.Low expression of ZNF545 was an independent risk factor for axillary lymph node metastasis in patients with TNBC(OR=2.355,95%CI 1.080-5.134,P=0.031).The expression level of ZNF545 was negatively correlated with the number of axillary lymph node metastases(r=-0.3241,P=0.013).Conclusion:Low expression of ZNF545,which may be involved in the process of TNBC by affecting Wnt/β-catenin signaling pathway related proteins,is an independent risk factor of prognosis and axillary lymph node metastasis in patients with TNBC.