生物样品中强心苷类药物的检验方法研究进展

Progress of analytical methods of cardiac glycosides in biological samples

生物样品中强心苷类药物检验需要高效的提取方法和高灵敏度仪器分析方法。本文从强心苷类药物的实验室检测和现场快速检测2个方面展开。其中,实验室检测包括蛋白沉淀、固相萃取、固相支撑-液液萃取、液-液微萃取、QuEChERS等样品前处理技术,以及液相色谱-质谱联用、实时直接分析-质谱联用、毛细管电泳等分析方法;现场快速检测包括免疫分析、生物传感器等分析方法。最后,本文对目前强心苷类药物分析和样本预处理方法的不足及发展前景做了初步探讨,旨在为法庭科学和临床治疗中强心苷类药物的检测提供技术参考。

The detection of cardiac glycosides in biological samples requires efficient extraction methods and high-sensitivity analytical methods.Among them,laboratory tests include protein precipitation,solid-phase extraction,solid-phase support-liquid-liquid extraction,liquid-liquid microextraction,QuEChERS and other sample pretreatment technologies,as well as liquid chromatography-mass spectrometry,real-time direct analysis-mass spectrometry,capillary electrophoresis and other analytical methods.Field rapid detection includes immunoassay,biosensor etc.Finally,this review makes a preliminary discussion on the shortcomings and development prospects of the current cardiac glycosides analysis and sample pretreatment methods.This review provides references for the detection of cardiac glycosides in forensic science and clinical treatment.