复方季铵盐消毒液对生物安全三级实验室新型冠状病毒消毒效果研究

Study on the effect of MCP disinfectant for SARS-CoV-2 in biosafety level 3 laboratory

目的 探讨在生物安全三级实验室(P3实验室)内以复方季铵盐消毒液(MCP)取代次氯酸钠作为新型冠状病毒(SARS-CoV-2)主要消毒剂的可行性。方法 配制体积百分浓度为2%、5%和100%的MCP以及含有效氯250、500或1 000 mg/L的次氯酸钠消毒液,采用凝胶过滤悬液定量消毒试验方法,在消毒剂与病毒液以体积比为1:2、1:1、2:1、5:1混合条件下分别作用1 min,测定MCP和次氯酸钠对SARS-CoV-2的灭活效果。结果 不同浓度和不同配比条件下的2种消毒液对悬液内的SARS-CoV-2灭活对数值均> 4.0。该MCP消毒液保存90 d,对新型冠状病毒的消毒效果无明显降低。结论 MCP消毒液和次氯酸钠消毒液对新型冠状病毒消毒效果良好,可用MCP替代次氯酸钠消毒液作为P3实验室有效消毒剂。

Objective To explore the feasibility of replacing sodium hypochlorite with compound quaternary ammonium salt disinfectants(MCP)as the main disinfectant for SARS-CoV-2 in a biosafety level 3 laboratory(P3 lab).Methods MCP with volume percentage concentrations of 2%,5%and 100%and sodium hypochlorite disinfectants containing 250,500 or 1000 mg/L of available chlorine were prepared,and the quantitative disinfection test of gel filtration suspension was used to test the inactivation effect of MCP and sodium hypochlorite on SARS-CoV-2 under the mixed condition of volume ratio of disinfectant to virus of 1:2,1:1,2:1,5:1 for 1 min.Results The inactivation logarithmic values of SARS-CoV-2 in the suspension of the two disinfectants with different concentrations and different ratios were all>4.0.The disinfection effect of the MCP disinfectant on SARS-CoV-2 was not significantly reduced after 90 d of storage.Conclusion MCP disinfectant and sodium hypochlorite disinfectants have good disinfection effects on SARS-CoV-2,and MCP can be used instead of sodium hypochlorite disinfectant in P3 lab as an effective disinfectant.