长链非编码RNA FAM83H-AS1对子宫内膜癌细胞增殖、侵袭和转移的影响

Effect of long non-coding RNA FAM83H-AS1 on proliferation,invasion,and metastasis of endometrial carcinoma cells

目的探讨长链非编码RNA序列相似性家族83成员H反义RNA 1(FAM83H-AS1)对子宫内膜癌细胞增殖、侵袭和转移的影响。方法收集2018年10月至2020年10月就诊于郑州人民医院的39例子宫内膜癌患者的肿瘤组织以及配对的癌旁组织。逆转录定量聚合酶链式反应(qRT-PCR)检测子宫内膜癌组织及癌旁组织中FAM83H-AS1的表达水平;用Lipofectamine2000转染试剂盒将靶向FAM83H-AS1的shRNA质粒转染至子宫内膜癌细胞HEC-1A和HEC-1B细胞中,通过qRT-PCR检测shRNA的转染效率。采用细胞增殖试验、TUNEL染色、EdU染色、流式细胞术以及Transwell试验等检测敲低FAM83H-AS1的子宫内膜癌细胞的生长增殖能力、凋亡水平以及迁移、侵袭能力。采用配对t检验和方差分析。结果癌组织长链非编码RNA FAM83H-AS1表达水平明显高于癌旁组织;长链非编码RNA FAM83H-AS1的表达水平与患者的年龄、肿瘤大小无明显的相关性(均P>0.05),与组织学类型、FIGO分期、淋巴结转移情况以及组织学分级有相关性(均P<0.05);敲低长链非编码RNA FAM83H-AS1削弱子宫内膜癌细胞的增殖活性,促进子宫内膜癌细胞的凋亡,抑制子宫内膜癌细胞的迁移与侵袭。结论长链非编码RNA FAM83H-AS1在子宫内膜癌组织中的表达水平明显增高;敲低FAM83H-AS1削弱子宫内膜癌细胞的增殖活性,促进子宫内膜癌细胞的凋亡,抑制子宫内膜癌细胞的迁移与侵袭。

Objective To explore the effect of long non-coding RNA(lncRNA)family with sequence similarity 83,member H antisense RNA1(FAM83H-AS1)on the proliferation,invasion,and metastasis of endometrial cancer cells.Methods The tumor tissue and adjacent tissue were collected from 39 patients with endometrial cancer who were treated at Zhengzhou People's Hospital from October 2018 to October 2020.Reverse transcriptase-quantitative polymerase chain reaction(qRT-PCR)was used to detect the epression levels in the endometrial cancer tissue and adjacent tissue.The shRNA plasmid targeting FAM83H-AS1 was transfected into the endometrial cancer cells HEC-1A and HEC-1B by the Lipofectamine 2000 transfection kit;the transfection efficiency of shRNA was detected by qRT-PCR.The cell proliferation test,TUNEL staining,EdU staining,flow cytometry,and Transwell test were used to detect the growth,proliferation,apoptosis,migration,and invasion abilities of the FAM83H-AS1 knockdown endometrial cancer cells.Paired t test and analysis of variance were applied.Results The expression level of lncRNA FAM83H-AS1 in the cancer tissue was significantly higher than that in adjacent tissue.The expression level of lncRNA FAM83H-AS1 was not significantly correlated with the patients'age and tumor size(both P>0.05),but was correlated with their histological type,FIGO stage,lymph node metastasis,and histological grade(all P<0.05).Knocking down lncRNA FAM83H-AS1 weakened the proliferation activity of the endometrial carcinoma cells,promoted the apoptosis,and inhibited the migration and invasion.Conclusions The expression level of lncRNA FAM83H-AS1 is significantly increased in endometrial cancer tissue.Knocking down FAM83H-AS1 weakens endometrial cancer cells'proliferative activity,promotes their apoptosis,and inhibits their migration and invasion.