长链非编码RNA MEG3在原发性肝癌中的表达及其意义

Expression and Significance of LncRNA MEG3 in Hepatocellular Carcinoma

【目的】探讨长链非编码RNA(LncRNA)MEG3在原发性肝癌(HCC)中的表达及其临床意义。【方法】本院收治的78例HCC患者均接受手术治疗,术中收集患者肝癌组织及癌旁正常组织;招募同期就诊于本科的肝血管瘤患者78例,收集术中标本。采用RT-PCR检测三种样本中LncRNA MEG3的表达水平并比较,评价LncRNA MEG3诊断HCC的价值,探讨LncRNA MEG3与HCC临床病理特征的相关性。【结果】LncRNA MEG3在肝癌组织中的表达为0.43±0.16,显著低于癌旁正常组织(1.18±0.35)及肝血管瘤组织(1.21±0.27)(均P<0.05);LncRNA MEG3诊断HCC的曲线下面积(AUC)(95%CI)为0.85(0.80~0.91),最佳诊断界值为0.91,敏感度89%,特异度76%;LncRNA MEG3与甲胎蛋白呈线性正相关(r=0.63,P=0.03);低表达LncRNA MEG3与HCC临床分期、分化程度以及远处转移有相关性(χ^(2)分别为3.93、6.59及4.43,均P<0.05)。【结论】LncRNA MEG3在HCC中呈低表达,低表达LncRNA MEG3与HCC病理特征有相关性,是潜在的HCC诊断指标及治疗靶点。

【Objective】To investigate the expression and value of LncRNA MEG3 in hepatocellular carcinoma(HCC).【Methods】A total of 78 patients who were diagnosed with HCC and underwent surgical treatment in our hospital were included in this study.The liver cancer tissues and adjacent normal tissues were collected during operation.At the same time period,78 patients who underwent surgical treatment of"hepatic hemangioma"in our department were enrolled and the intraoperative specimens of them were collected.RT-PCR was used to detect the expression level of LncRNA MEG3 in all subjects and the difference of LncRNA MEG3 levels was compared among different groups.The value of LncRNA MEG3 for diagnosis of HCC was analyzed and the correlation of LncRNA MEG3 with clinical pathological of HCC and alpha fetoprotein(AFP)was studied as well.【Results】The expression level of LncRNA MEG3 in HCC tissues was 0.43±0.16,which was lower than that of adjacent normal tissues(1.18±0.35)and hepatic hemangioma(1.21±0.27)(P<0.05).The area under the value curve(AUC)(95%CI)of LncRNA MEG3 for the diagnosis of HCC was 0.85(0.80-0.91),and the best diagnostic cutoff value was 0.91,the sensitivity was 89%and the specificity was 76%.LncRNA MEG3 had a linear positive correlation with AFP(r=0.632,P=0.030).The low expression of LncRNA MEG3 had a correlation with HCC clinical stage,differentiation and distant metastasis of liver cancer(χ2 were 3.93,6.59 and 4.43,respectively,P<0.05).【Conclusion】LncRNA MEG3 has low expression in HCC,and it is closely related to the clinicopathology of HCC,which can be severed as a potential target for diagnosis and treatment of HCC.