母婴PFASs靶向与非靶向分析及新生儿健康风险

Target and nontarget analysis of PFASs in mothers and newborns and related health risks of newborns

对采集自阜新氟化工厂周边产妇50组配对母体、新生儿血清以及胎盘样本,进行靶向分析与非靶向筛查.在靶向分析中,采用高效液相色谱-串联质谱(HPLC-MS/MS)对所有样品中的21种传统全氟和多氟烷基物质(PFASs)进行定量分析,发现全氟辛酸(PFOA)、全氟丁酸(PFBA)和全氟丁烷磺酸(PFBS)为最主要的PFASs污染物.通过基于高分辨率质谱(HRMS)的非靶向筛查,在所有样本中共识别出11类(49种)新型PFASs,其中4类(20种)新型PFASs首次在人体血清和胎盘中发现.传统PFASs母体-胎盘转移(R_(m/p))和母体-新生儿转移(R_(m/n))系数的中位数分别为1.7和2.0;而新型PFASs的R_(m/p)和R_(m/n)中位数分别为0.9和0.8.从而导致新型PFASs从母体血清到新生儿血清的贡献率由90%增加至96%.此外,线性回归分析揭示新生儿生长参数(妊娠期和头围)、血清中甲状腺激素、性激素和糖皮质激素水平与新生儿血清中若干传统和新型PFASs水平之间存在显著相关性,其中最具代表性的化合物为全氟羧酸类化合物(PFCAs)、全氟磺酸类化合物(PFSAs)、氢代全氟羧酸类化合物(H-PFCAs)、氢代全氟磺酸类化合物(H-PFSAs)和氯代多氟醚磺酸类化合物(Cl-PFESAs).血清中传统和新型PFASs可能对新生儿造成一定的健康风险.

50 paired samples of maternal and newborn serum,as well as placenta were collected from pregnant women at delivery around Fuxin fluorochemical facilities,and all the samples were analyzed through the target and non-target screening strategies.In the target analysis,21 legacy per-and polyfluoroalkyl substances(PFASs)in the whole samples were analyzed through high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS),revealing that perfluorobutane sulfonate(PFBS),perfluorobutanoic acid(PFBA),and perfluorooctanoic acid(PFOA)were the dominant PFAS contaminants observed in all the samples.In the suspect screening based upon high-resolution mass spectrometry(HRMS),49 novel PFASs in 11classes were identified in the Fuxin samples,and 20 novel congeners assigned to 4 classes were determined in human sera and placentas for the first time.In addition,the coefficients for mother-placenta transfer(R_(m/p))and mother-newborn transfer(R_(m/n))of legacy PFASs could be estimated with the median values of 1.7 and 2.0 individually,and R_(m/p)and R_(m/n)of novel PFASs were also calculated with the median values of 0.9 and 0.8,respectively.Accordingly,the average contribution rate of all novel PFASs increased from 90%in maternal serum to 96%in neonatal serum.Moreover,the linear regression analysis determined significant correlations among the neonatal growth parameters(gestation and head circumferences of newborns)and serum levels of thyroid hormone,sex hormone,and glucocorticoid,together with the levels of certain legacy and novel PFASs in neonatal sera,and the most representative compounds included perfluorocarboxylic acids(PFCAs),perfluorosulfonic acids(PFSAs),H-substituted perfluorocarboxylic acids(H-PFCAs),H-substituted perfluorosulfonic acids(H-PFSAs),and chlorinated polyfluorinated ether sulfonates(Cl-PFESAs).The results above demonstrated that legacy and novel PFASs could potentially contribute to certain health risks of newborns.