甜叶菊提取物对高糖脂饮食鼠血脂及其肠道菌群的影响

Effects of stevia extract on blood lipids and intestinal flora in mice fed with high sugar and fat diet

探究甜叶菊提取物对脂质代谢、氧化应激和肠道微生态的影响。选取健康昆明鼠,构建脂代谢紊乱模型,分析其血脂、氧化应激及其肠道菌群变化。甜叶菊提取物显著降低了血脂(甘油三酯、胆固醇、低密度脂蛋白和游离脂肪酸)含量,显著提高了血清中抗氧化酶[超氧化物歧化酶(superoxide dismutase, SOD)和谷胱甘肽氧化物酶(glutathione peroxidase, GSH-Px)]水平,降低了丙二醛含量。病理组织切片显示,甜叶菊提取物显著改善了肝脏和结肠组织的损伤。高通量测序结果显示提取物提高了肠道拟杆菌水平,降低了厚壁菌水平。甜叶菊提取物能够调节脂质代谢,维持氧化应激平衡,抑制肝脏脂质堆积,同时具有益生元活性。

To investigate the effects of stevia extract on lipid metabolism,oxidative stress and intestinal microecology.We chose healthy Kunming mice,built models of lipid metabolic disorders,and examined changes in blood lipids,oxidative stress,and intestinal flora.Stevia extract significantly reduced serum lipids indices(triglycerides,cholesterol,low-density lipoprotein,and free fatty acids),considerably increased serum levels of antioxidant enzymes(superoxide dismutase and glutathione peroxidase),and decreased aldehyde content.Histopathological observation showed that stevia extract significantly reduced liver and colon tissue damage,such as data from high-throughput sequencing showed the stevia extract lowered the number of Firmicutes and raised the number of Bacteroidetes in the intestine.Stevia extract has prebiotic effect and can control lipid metabolism from anomalies,oxidative stress from out-of-balance,and liver fatty deposition.