CD133在胃肠道间质瘤中的表达及临床意义

Expression and clinical significance of CD133 in gastrointestinal stromal tumors

目的探讨胃肠道间质瘤(GIST)CD133蛋白的表达及其与临床病理特征。方法采用免疫组织化学法检测363例GIST组织中CD133的表达情况,并分析与临床病理指标的相关性。结果CD133主要在细胞膜表达,可少量表达于细胞质,染色黄色或棕黄色细胞代表阳性,细胞核为蓝色,底物呈现白色;CD133的表达量与肿瘤直径、危险分度以及肿瘤位置差异有统计学意义(P<0.05);GIST肿瘤位置、肿瘤直径、核分裂象、危险分度、肿瘤是否转移、Ki-67的表达与生存预后之间存在相关性(P<0.05),而CD133表达量的高低与GIST患者预后无关(P=0.717)。Cox多因素分析提示GIST肿瘤位置、Ki-67>5%以及CD133高表达是影响患者预后的独立危险因素(P<0.05)。结论CD133表达量在不同肿瘤大小、核分裂象及肿瘤是否转移的GIST患者中存在差异,提示CD133可能参与了肿瘤的增殖与迁移的调控,从而加速肿瘤的生长与转移;CD133高表达是GIST预后的独立危险因素。

Objective To investigate the expressions of CD133 protein in gastrointestinal stromal tumor(GIST)and its relationship with clinicopathological characteristics.Methods A total of 363 GIST patients were collected.The CD133 expression in GIST was detected by using immunohistochemical method.The relationship between CD133 expression and clinicopathological parameters was analyzed.Results CD133 was mainly expressed in the cell membrane,but a small amount in the cytoplasm.The stained yellow or brown cells represented positive,the nucleus was blue,and the substrate was white.The expression of CD133 was significantly different in different tumor diameter,risk scale and tumor location(P<0.05).There was a correlation between GIST tumor location,tumor diameter,mitotic figures,risk scale,tumor metastasis,KI-67 expression and survival prognosis(P<0.05),while the level of CD133 expression was not related to the prognosis of GIST patients(P=0.717).Cox multivariate analysis showed that non-gastric GIST,Ki-67>5%,and high expression of CD133 were independent risk factors affecting the prognosis of patients(P<0.05).Conclusions The expression of CD133 is different in GIST patients with different tumor size,mitosis and metastasis.This suggests that CD133 may participate in the regulation of tumor proliferation and migration.Thus,it would accelerate tumor growth and metastasis.The high expression of CD133 is an independent risk factor for the prognosis of patients with GIST.