活血解毒汤对胃癌前病变小鼠细胞黏附分子的影响

Effects of Huoxue Jiedu Decoction on cell adhesion molecules in mice with precancerous gastric cancer

目的观察活血解毒汤对小鼠胃癌前病变的逆转作用,并从细胞黏附分子角度探讨其作用机制。方法将50只昆明小鼠随机分为对照组、模型组及高、中、低剂量中药组共5组,每组各10只。对照组正常饲养,其余各组采用甲基硝基亚硝基胍(MNNG)溶液自由饮用加灌胃法建立胃癌前病变(PLGC)模型。造模成功后,高、中、低剂量中药组分别给予不同剂量(15.86、31.72、63.44 g/kg)活血解毒汤灌胃治疗,每日1次,持续60 d。结果与对照组相比,模型组小鼠体质量明显降低,胃液p H值显著升高(P<0.01),胃黏膜萎缩,胃腺数量减少,可见异型增生,血清ICAM-1、CD44水平显著升高,JAM-1的水平明显降低(P<0.01),胃黏膜组织中ICAM-1、CD44的阳性表达明显增多,JAM-1的阳性表达明显减少(P<0.01);高、中、低剂量中药组干预60 d后,小鼠体质量较模型组明显增高,胃液p H值明显降低(P<0.05,P<0.01),胃黏膜组织萎缩和异型程度明显改善,明显降低了血清ICAM-1、CD44水平,升高了JAM-1的水平(P<0.05,P<0.01),同时显著下调了胃黏膜组织中ICAM-1、CD44的阳性表达,上调了JAM-1的阳性表达(P<0.05,P<0.01)。结论活血解毒汤对PLGC模型小鼠具有较好的治疗效果,可有效逆转小鼠胃黏膜病变,抑制异型性细胞增生,其机制可能与调节细胞黏附分子的表达相关。

Objective To observe the reversing effect of Huoxue Jiedu Decoction on the precancerous lesions of gastric cancer(PLGC)in mice,and to explore its mechanism from the perspective of cell adhesion molecules.Methods Fifty Kunming mice were randomly divided into 5 groups:a control group,a model group,and high-,medium-and low-dose Chinese medicine groups,with 10 mice in each group.The control group was fed normally,and the other groups were treated with the methyl nitro nitroso guanidine(MNNG)solution for free drinking and intragastric administration to establish the model of precancerous gastric cancer.After it,the high-,medium-and low-dose Chinese medicine groups were treated with different doses(15.86,31.72,and 63.44 g/kg)of Huoxue Jiedu Decoction by gavage once a day for 60 days.Results Compared with the control group,the mice body weight of the model group was significantly decreased,the pH value of gastric juice was significantly increased(P<0.01),the gastric mucosa was atrophied,the number of gastric glands was reduced,dysplasia was observed,the levels of serum ICAM-1 and CD44 were significantly increased,the level of JAM-1 was significantly decreased(P<0.01),the positive expression of ICAM-1 and CD44 in gastric mucosa was significantly increased,while the positive expression of JAM-1 was significantly decreased(P<0.01).After 60 days of intervention,the body weight of mice in the high-,mediumand low-dose Chinese medicine groups was significantly higher than that in the model group,the pH value of gastric juice was significantly decreased(P<0.05,P<0.01),the atrophy and dysplasia of gastric mucosa were significantly improved,and the levels of serum ICAM-1 and CD44 were significantly reduced,while the level of JAM-1 was increased(P<0.05,P<0.01),and the positive expression of ICAM-1 and CD44 in gastric mucosa was significantly down-regulated,while the positive expression of JAM-1 was up-regulated(P<0.05,P<0.01).Conclusion Huoxue Jiedu Decoction has a significant therapeutic effect on PLGC model mice,which can effectively reverse the pathological changes of gastric mucosa in mice and inhibit the heterotypic cell proliferation.Its mechanism may be related to the regulation of the expression of cell adhesion molecules.