基于低分子量PEI的不同疏水链修饰的梳状低聚物基因载体

Comblike Oligomers Modified with Different Hydrophobic Chain Based on Low Molecular Weight PEI Were Used as Gene Vectors

为了探究基于低分子量聚乙烯亚胺(polyethylenimine,PEI)基因载体的转染效率,通过Michael加成反应将PEI 600 Da接枝于含有疏水链的生物可降解聚酯上形成系列梳状聚合物,并将之应用于基因载体;利用核磁氢谱和凝胶渗透色谱对聚合物的化学结构与分子量进行了测定,此外,还利用凝胶电泳实验和绿色荧光蛋白实验研究了聚合物与DNA的结合能力及其复合物的转染性能。结果表明:本文方法成功合成了系列低聚物,并对DNA表现出较好的包裹能力;油酸修饰后的低聚物与DNA复合物在质量比为6.4时转染效果与PEI 25 kDa相当。可见,油酸修饰后的脂质体低聚物有作为非病毒基因载体的前景。

In order to study the transfection effect of gene vector based on low molecular weight polyethylenimine(PEI),Michael addition reaction was used to graft PEI 600 Da onto biodegradable polyesters containing hydrophobic chains to form a series of comblike polymers,and then they were applied to the gene vector.The chemical structure and molecular weight of the polymers were determined by utilizing 1 HNMR and gel permeation chromatography respectively.Additionally,the binding ability of the polymers and DNA,the transfection performance of their complexes were also studied by use of gel electrophoresis and green fluorescent protein assay.The results show that a series of oligomers are successfully synthesized,by which DNA can be packaged well.The transfection effect of oleic acid-modified oligomer/DNA complex is similar to that of PEI 25 kDa at mass ratio 6.4.It is concluded that oleic acid-modified liposome oligomer has the potential to be used as non-viral gene vector.