多花黄精多酚工艺条件优化及其抗氧化活性评价

Optimization of Process Conditions and Evaluation of Antioxidant Activity of Polyphenols from Polygonatum Multiflorum

建立微波辅助提取多花黄精多酚提取方法。以多酚得率作为指标来考察料液比、乙醇浓度、微波功率、微波提取时间等因素对多酚提取的影响,确定多花黄精单因素试验;在单因素的基础上设计响应面试验获得最优多酚提取工艺条件。在最佳提取工艺条件下:80%乙醇、810 W微波功率、8 min提取时间、1∶30 g/mL料液比,多酚得率为11.06 mg/g。多花黄精多酚对羟基自由基率达到13.80%,对牛血清白蛋白具有一定的荧光淬灭作用达到12.50%。

Microwave-assisted extraction of polyphenols from polygonatum multiflorum was established.Polyphenol yield was used as an index to study the effects of material-liquid ratio,ethanol concentration,microwave power and microwave extraction time on polyphenol extraction based on the single factor,the response surface methodology was designed to obtain the optimal polyphenol extraction conditions.Under the optimum extraction conditions as 80%ethanol,810 W microwave power,8 min extraction time,1∶30 g/mL ratio of material to liquid,the yield of polyphenol was 11.06 mg/g.Polygonatum multiflorum polyphenols had a fluorescence quenching effect of 12.50%on bovine serum albumin and 13.80%on hydroxyl radical.