T_(2)W-DRIVE-HR序列在钆造影核磁共振成像检测豚鼠实验性内耳内淋巴积水中的应用

The Application of T_(2)W-DRIVE-HR Sequence in Gadolinium Contrast Magnetic Resonance Imaging in the Detection of Experimental Endolymphatic Hydrops in the Inner Ear of Guinea Pigs

目的研究驱动平衡自旋回波序列(T_(2)W-DRIVE-HR)在钆造影核磁共振成像(MRI)时检测豚鼠内耳内淋巴积水的效果。方法8只雌性豚鼠分为两组,包括对照组和内淋巴积水模型组(腹腔注射醋酸去氨加压素),各组相应处理17天后进行内耳磁共振成像和组织形态学检查。结果T_(2)W-DRIVE-HR序列在矢状位扫描时内耳成像效果好。内耳磁共振成像结果表明,与对照组相比,积水模型组豚鼠耳蜗内淋巴体积明显增加(t=3.594,P<0.05);组织形态学结果表明,与对照组相比,积水模型组豚鼠耳蜗每一转中阶面积/(前庭阶面积+中阶面积)的比值明显升高(t=3.105,P<0.05)。结论腹腔注射醋酸去氨加压素诱导豚鼠内耳内淋巴积水,使用T_(2)W-DRIVE-HR序列在钆造影磁共振成像检测的内耳内淋巴积水与组织形态学检测结果一致,说明该序列可用于豚鼠内耳内淋巴积水的检测。

Objective To study the effect of driving balanced spin echo sequence(T_(2)W-DRIVE-HR)in detecting endolymphatic hydrops in the inner ear of guinea pigs during gadolinium contrast magnetic resonance imaging(MRI).MethodsEight female guinea pigs were divided into control group and endolymphatic hydrocephalus model group(intraperitoneal injection of desmopressin acetate).Magnetic resonance imaging and histomorphology examinations of the inner ear were performed after 17 days of treatment in each group.Results T_(2)W-DRIVE-HR sequence has better imaging effect in sagittal scanning of inner ear.The MRI images of the inner ear showed that the volume of endolymph in the cochlea was higher in the hydrops model group than in the control group(t=3.594,P<0.05).The histomorphology of inner ear showed that the ratio of media scale area to media scale and vestibular scale area was increased in the hydrops model group compared with the control group(t=3.105,P<0.05).Conclusion Intraperitoneal injection of desmopressin acetate can induce endolymphatic hydrops in guinea pigs.The results of endolymphatic hydrops in the inner ear using T_(2)W-DRIVE-HR sequence are consistent with histomorphology.Thus,this sequence can be used for the measurement of endolymphatic hydrops.