富含羟基酪醇橄榄油对衰老小鼠的影响

Effect of olive oil containing hydroxyl tyrosol on aging mice

【目的】为研究富含羟基酪醇橄榄油(Olive oil rich in hydroxyl tyrosol,OHT)对衰老小鼠的影响。【方法】利用小鼠颈部皮下注射500 mg/kg的D-半乳糖(D-galactose)溶液构建亚急性衰老模型,建模成功后,将小鼠随机分成7组,分别为空白对照组(CK_(0))、模型组(Model group)、维生素E阳性对照组(VE positive control group)、普通橄榄油组(Cormal olive oil group)和OHT高、中、低剂量(200、100、50 mg/kg)处理组(OHT high,medium,low-dose group),连续灌胃28 d,检测小鼠体质量变化及脏器指数、血清中谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)活性及丙二醛(malondialdehyde,MDA)含量的变化,观察肝组织形态学变化。【结果】OHT使衰老小鼠的体质量和脏器指数显著升高(P<0.05,P<0.01),肝脏病理变化得到改善,血清CAT、GSH-Px和SOD活性显著升高(P<0.05,P<0.01),MDA含量显著降低(P<0.05,P<0.01),以上指标变化在OHT高剂量组尤为明显,且OHT干预效果强于普通橄榄油。【结论】OHT能提高机体抗氧化能力,进而发挥抗衰老的作用。

【Objective】To study the effects of olive oil containing hydroxyl tyrosol(OHT)on aging mice.【Method】An aging animal model was established by subcutaneous injection of 500 mg/kg Dgalactose in the neck,and after successful modeling,the mice were randomly divided into 7 groups:blank control group,model group,VE positive control group,common olive oil group,OHT high,medium and low dose treated groups(200 mg/kg,100 mg/kg,50 mg/kg),the OHT was administered to the mice by intragastric administration for 28 consecutive days.The weight and viscera indexes of each group were calculated,and the activities of glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),catalase(CAT)and malondialdehyde(MDA)in the serum were determined,and the morphology changes of the liver tissue were observed.【Result】OHT could significantly improve the body weight and organ index in aging mice(P<0.05,P<0.01),ameliorate the pathological changes of the liver,significantly increase the activity of serum CAT,GSH-Px and SOD(P<0.05,P<0.01),and significantly reduced MDA content(P<0.05,P<0.01).The changes in the above indexes were particularly evident in the high-dose OHT group,and the intervention effect of OHT was stronger than that of normal olive oil.【Conclusion】OHT could improve the body’s antioxidant capacity to play an anti-aging role.