河西地区玉米田棉铃虫对2种Bt蛋白的敏感性监测

Sensitivity of cotton bollworms to two types of Bt proteins in cornfield of Hexi Corridor

【目的】明确河西走廊棉铃虫对玉米Bt蛋白的敏感性,为科学监测棉铃虫的发生危害规律提供科学依据。【方法】饲料表面覆盖毒素法测定了河西走廊嘉峪关(JYG)、酒泉(JQ)、张掖(ZY)、武威(WW)4个棉铃虫地理种群对Cry1Ab和Cry1Ac蛋白的敏感性,计算LC_(50)和LC_(99).【结果】JYG、JQ、ZY和WW种群对Cry1Ab的LC_(50)分别为0.1162、0.1460、0.1721、0.2004μg/cm^(2),LC_(99)分别为7.2778、6.0807、97.0666和27.1037μg/cm^(2);JYG、JQ、ZY和WW地理种群对Cry1Ac的LC_(50)分别为0.1050、0.2554、0.0172和0.0456μg/cm^(2),LC_(99)分别为0.1598、11.7953、1.1904、7.4709μg/cm^(2),其中JQ地理种群对Cry1Ac的LC_(50)值显著高于其他地理种群.【结论】河西走廊玉米棉铃虫对Cry1Ab和Cry1Ac蛋白的敏感性较高,暂未对Cry1Ab和Cry1Ac蛋白产生抗性。但应该持续加强监测,以避免Bt交互抗性产生应用风险.

【Objective】The study aimed to determine the sensitivity of Bt proteins to the corn bollworm of Hexi Corridor,laying a scientific basis for monitoring the occurrence and damage of cotton bollworm.【Method】The susceptibility of four geographical populations of cotton bollworm in JYG,JQ,ZY,and WW in Hexi Corridor to Cry1Ab and Cry1Ac proteins was determined using Diet-overlay bioassays,and LC_(50)and LC_(99)were calculated.【Result】The results showed that the LC_(50)of Cry1Ab protein in JYG,JQ,ZY,and WW geographic populations were 0.1162,0.1460,0.1721,and 0.2004μg/cm^(2),respectively,while the LC_(99)of Cry1Ab were 7.2778,6.0807,97.0666,and 27.1037μg/cm^(2),respectively.The LC_(50)of Cry1Ac protein in JYG,JQ,ZY,and WW geographic populations were 0.1025,0.2554,0.0172,and 0.0456μg/cm^(2),respectively,while the LC_(99)of Cry1Ac were 0.1598,11.7935,1.1904,and 7.4709μg/cm^(2),respectively.The LC_(50)of Cry1Ac in the JQ geographic population was significantly higher than that of other geographic populations.【Conclusion】In conclusion,the corn bollworm of Hexi Corridor is highly sensitive to Cry1Ab and Cry1Ac proteins,and has not yet developed resistance to them.However,monitoring should be continuously strengthened to avoid the creation of application risks due to cross-resistance.