响应面优化石榴皮多酚提取物提取工艺及其活性研究

Studies on extraction process optimization and activities of polyphenol matter from pomegranate peel by response-surface methodology

【目的】优化从石榴皮中提取多酚的工艺,并探究3种常见石榴皮提取物(会理水晶石榴(HL)、突尼斯软子石榴(TN)、云南蒙自甜石榴(MZ))的组成成分和抗氧化抑菌活性。【方法】采用单因素试验、响应面分析法研究提取时间、乙醇浓度、温度、料液比对总酚含量的影响并确定最优提取工艺,核磁共振法分析石榴皮提取物成分,通过抗氧化抑菌试验,评估其抗氧化抑菌能力。【结果】最佳提取条件为:提取时间77 min、乙醇浓度36.0%、温度66.9℃、料液比1∶37。在此条件下,总酚含量为(242.026±1.94)mgGAE/g,。石榴皮提取物的主要成分为糖类、有机酸、酚类化合物等物质。抗氧化能力依次为HL>TN>MZ>丁基羟基茴香醚。3种细菌对石榴皮提取物的敏感度依次为:荧光假单胞菌>金黄色葡萄球菌>大肠杆菌,抗菌能力依次为HL>TN=MZ。【结论】HL的抗氧化抑菌能力最强,研究结果为石榴皮提取物提取及利用提供了实验依据。

【Objective】The study aimed to optimize extraction process of polyphenols from pomegranate peel,and to investigate the components of three common extracts(Huili crystal pomegranate(HL),Tunisian soft pomegranate(TN),Yunnan Mengzi sweet pomegranate(MZ))and their antioxidant and antibacterial activities.【Method】Single factor test were designed to study the effects of extraction time,ethanol concentration,temperature and solid-liquid ratio on total phenol content through response-surface analysis,followed by determining optimal extraction process.Nuclear magnetic resonance was used to analyze the components of pomegranate peel extracts,and the antioxidant and bacteriostatic abilities of the extracts were evaluated by antioxidant and bacteriostatic test.【Result】The optimal extraction conditions were as follows:extraction time for 77 min,ethanol concentration of 36.0%,temperature at 66.9°C,solid-liquid ratio of 1∶37.Under these conditions,the total content of phenols was(242.026±1.94)mgGAE/g.The main components of pomegranate peel extract were sugars such as pectin,organic acids,phenolic compounds,etc.The antioxidant capacity of these extracts ranged as follows:HL>TN>MZ>butylated hydroxyanisole.The susceptibility of the three bacteria to pomegranate peel extract ranged in the following order:Pseudomonas fluorescens>Staphylococcus aureus>Escherichia coli,and the antibacterial ability ranged in the order of HL>TN=MZ.【Conclusion】The antioxidant and bacteriostatic capacity of HL was the strongest,and the results laid an experimental basis for extraction and utilization of pomegranate peel extract.