Exploration of anti-cardiac fi brosis active ingredients of Eucalyptus globulus Labill. fruit and their mechanism of action based on chromatography-mass spectrometry and network pharmacology

The fruit of Eucalyptus globulus Labill.,also known as“Yikouzhong(YKZ)”in China,belongs to the myrtaceae family.The aim of this study was to characterize the chemical composition of YKZ extract by GC-MS combined with LC-MS/MS.A total of 34 compounds were initially identified by GC-MS and 54 compounds were identified by LC-MS/MS positive and negative total ion flow maps.The total phenols and flavonoid-like substances were quantified using an enzyme standardization method to determine the presence of phenolic substances in them.At the same time,a network pharmacology approach based on LC-MS/MS was used to construct a“drug-component-target”network for myocardial protection,and 696 potential anti-myocardial fibrosis targets were obtained.The PPI analysis identified 72 core targets,and the enrichment analysis revealed that the core targets may exert anti-myocardial fibrosis effects by regulating Lipid and atherosclerosis and Proteoglycans signaling pathways.Taken together,these results suggest that YKZ anti-cardiac fibrosis may act through multiple components and targets,and this study provides a scientific basis for the functional development and application of YKZ against myocardial fibrosis.