基于BODIPY的荧光探针检测多西环素及作用机制研究

BODIPY-based fluorescent probe for doxycycline detection and mechanism of action

采用一锅法合成了一种基于氟硼二吡咯(BODIPY)的荧光探针(DOX-BOD),通过~1H、~(13)C和HRMS对其结构进行表征,利用紫外/荧光分光光度法对DOX-BOD的性能进行研究。结果表明,在PBS溶液中,DOX-BOD对多西环素(doxycycline,DOX)表现出明显的荧光猝灭响应,猝灭率高达90%,检测限为0.89μmol·L^(-1),证明DOX-BOD对DOX的识别检测具有较高的灵敏度和抗干扰能力。此外,DOX-BOD还表现出优异的pH稳定性和光稳定性。通过紫外-可见光吸收光谱和Stern-Volmer方程分析,得出DOX-BOD与DOX的作用机制属于动态猝灭。基于BODIPY的荧光探针体系的构建,实现了对DOX的快速、高灵敏检测,有利于环境监测与评估,对人和动物的健康保障具有积极意义。

A BODIPY-based fluorescent probe(DOX-BOD)was synthesized by one-pot method,and its structure was characterized by 1 H,13 C and HRMS.The performance of DOX-BOD was studied by UV/fluorescence spectrophotometry.The results showed that DOX-BOD exhibited obvious fluorescence quenching response to doxycycline(DOX)in PBS solution,the quenching rate was as high as 90%,and the detection limit was 0.89μmol·L^(-1).It was proved that DOX-BOD had high sensitivity and anti-interference ability for the recognition and detection of DOX.In addition,DOX-BOD exhibited excellent pH stability and photostability.Through the a-nalysis of ultraviolet-visible absorption spectroscopy and Stern-Volmer equation,it is concluded that the mechanism of action of DOX-BOD and DOX belongs to dynamic quenching.The construction of the fluorescent probe system based on BODIPY has realized the rapid and highly sensitive detection of DOX,which is conducive to environmental monitoring and evaluation,and has positive significance for the health protection of humans and animals.