摘要
Insufficient bionic performance is a structural obstacle and makes urethral repair unobtainable.To overcome this challenge,we mimicked the urethral matrix and applied two electrospinning techniques to build a double-layer sponge tube of nanofib-ers and nanoyarns.Intriguingly,silk fibroin(SF)and vitamin B5(VitB5)could be introduced to increase the elasticity of the outer layer and reduce the hydrophobicity to further improve mesenchymal cell proliferation.Systematic experiments validated the bionic structure,biocompatibility,and exosome delivery capacity in this scaffold.We achieved scarless urethral repair by delivering the bioactive growth factors from adipose-derived stem cell exosomes by physical absorption.Biological regeneration of the urethra can be accomplished with continuous epithelium in animals.Furthermore,bioinformatics studies revealed that the expression of cell proliferation and fibrotic genes(e.g.,Wnt7a,cfa-miR-574)was responsible for the bio-logical regeneration of the adipose-derived stem cells exosomes(ADSC-exos)by delivering poly l-lactide-co-caprolactone/SF/VitB5 bilayer sponge(PSVBS)via reduced fibrosis gene expression,as well as improved epithelial formation and blood vessel formation.Therefore,the PSVBS design appeared to be an instructive approach for urethral and other tubular organ regeneration.
基金
National Natural Science Fund of China(Grant no.82170694,81700590)
the Shanghai Jiao Tong University Biomedical Engineering Cross Research Foundation(Grant no.YG2022ZD020,YG2017QN15)
Shanghai health committee(XHLHGG20,20184Y0053)
Shanghai natural science foundation(20ZR144210)
iangsu Key R&D Plan(BE2017664)
Shanghai"Rising stars of medical talent"Youth development program,Shanghai Jiao Tong University K.C.Wong Medical Fellowship Fund.The Talent Program of Shanghai University of Engineering Science(QNTD202104)
Shanghai Local Universities Capacity Building Project of Science and Technology Innovation Action Program(21010501700).
