附着生物膜的微塑料对黄孢原毛平革菌的毒性影响

Toxicity Eff ects of Biofi lm-covered Microplastics on Phanerochaete Chrysosporium

本文以聚乙烯和聚乳酸为目标污染物,黄孢原毛平革菌作为白腐真菌的代表。探究原始微塑料和附着生物膜的微塑料对黄孢原毛平革菌的毒性影响,其中包括活性分析以及抗氧化应激分析。结果表明微塑料能抑制黄孢原毛平革菌的活性,且附着生物膜的微塑料对其抑制程度更大。黄孢原毛平革菌在微塑料的胁迫下会产生氧化应激反应,在短期内,超氧化物歧化酶、过氧化氢酶和过氧化物酶的活性显著提高,存在协同作用;长期条件下反而会抑制它们的酶活。

In this study,polyethylene and polylactic acid were used as target contaminants and phanerochaete chrysosporium as a represen tative of white rot fungi.The toxic effects of virgin microplastics and biofilm-covered microplastics on phanerochaete chrysosporium were investigated,which included activity analysis as well as anti-oxidative stress analysis.The results showed that the microplastics inhibited the activity of phanerochaete chrysosporium,and the biofilm-covered microplastics inhibited the activity to a greater extent.Under the stress of microplastics,phanerochaete chrysosporium could produce oxidative stress response.In the short term,the activities of superoxide dismutase,catalase and peroxidase were significantly increased and there were synergistic effects.On the contrary,under long-term conditions,their enzymatic activities were inhibited.