动物肠类器官培养技术

Animal Intestinal Organoids Culture

类器官被定义为三维(three-dimensional,3D)多细胞体外自组装组织结构,在细胞类型、结构和功能方面能模拟还原相应的体内器官,具有明显的组织特异性,可作为新型体外模型。随着技术的发展,肠类器官已被应用于器官发育、器官功能、器官修复与移植、疾病诊断和药物筛选等多个研究领域。目前除了小鼠肠类器官外,关于其他种属动物肠类器官培养的研究较少。但在临床中,其他动物,特别是家畜动物仍存在多种传染性或非传染性的肠道疾病,亟待新型的体外模型用于揭示致病机理或开发新的治疗方案。为此,本文对近十年动物肠类器官培养的研究进展进行综述,总结了动物肠类器官培养的优、缺点并对动物肠类器官培养进行了小结与展望,以期为动物肠类器官的培养提供参考。

Organoids are defined as three-dimensional(3D)multicellular self-assembled tissue structures in vitro,which could simulate corresponding organs in vivo in terms of cell types,structural architecture and function.They have obvious tissue specificity and can be used as a novel experimental model in vitro.With the development of technology,organoids have been applied in many fields including organ morphogenesis,organ function,organ repair and transplantation,disease diagnosis,drugs screening,etc.Currently,other than murine intestinal organoid culture,only few studies on animal intestinal organoid culture were reported.However,other animals,especially domestic animals,still suffer from infectious and non-infectious intestinal diseases that urgently need a new study model for pathogenesis or new therapy.Therefore,this research progress focused on animal intestinal organoids culture in the last ten years with the advantages and the disadvantages of it were being summarized.This review may provide new ideas for the related research of animal intestinal organoids.