基于UPLC-Q-TOF-MS/MS代谢组学研究土霉素和穿心莲内酯对鸡代谢的影响

Effect of Oxytetracycline and Andrographolide on the Metabolism of Chicken Intestinal Tracts Using UPLC-Q-TOF-MS/MS-based Metabolomic Approach

本研究旨在建立基于色谱-质谱联用技术的代谢物分析技术平台,利用等剂量中兽药穿心莲内酯和广谱抗生素土霉素对3周龄仔鸡进行干预,分析药物干预对鸡的排泄物代谢变化,通过数据统计分析寻找差异性代谢物,探讨穿心莲内酯和土霉素对鸡肠道菌群代谢的干预机制。采用UPLC-Q-TOF-MS/MS检测各组黄羽鸡排泄物样本,通过MetaboAnalyst平台进行代谢通路的富集与分析,运用QI软件筛选出差异代谢物。结果表明,相较于空白组,土霉素与穿心莲内酯组分别存在12个和10个差异化合物,土霉素组涉及15条代谢通路,其中,2条最相关代谢通路分别为亚油酸代谢和甘油磷脂代谢的通路;穿心莲内酯组涉及17条代谢通路,其中,5条最相关代谢通路分别为甘油磷脂的代谢、鞘磷脂代谢、半胱氨酸和蛋氨酸的代谢、不饱和脂肪酸的生物合成以及甘氨酸、丝氨酸和苏氨酸的代谢通路。综上,土霉素和穿心莲内酯影响鸡代谢的作用机制十分相似,均可通过改变磷脂代谢、脂肪酸代谢、氨基酸代谢等途径对鸡正常代谢功能产生影响,尤其是土霉素组与对照组的分布规律有明显差异。

This experiment was conducted to establish a technical platform for metabolite analysis based on chromatography-mass spectrometry(UPLC-Q-TOF-MS/MS),three-week-old chickens were feed with equal dose of the Chinese veterinary drug andrographolide(AG)and the broad-spectrum antibiotic oxytetracycline(OTC)respectively,to analyze the metabolite changes in the excretion of chickens after intervention.Through statistical analysis of the data,differential metabolites were obtained,and the intervention mechanism of AG and OTC on chicken gut microbiota metabolism were also explored.The excretory samples of yellow-finned chickens in each group were detected by UPLC-Q-TOF-MS/MS,and the metabolic pathways were enriched and analyzed by MetaboAnalyst platform.The differential metabolites were screened by using QI software.The results showed that compared with the blank group,12 and 10 differential compounds were found in OTC and AG group,respectively.Fifteen metabolic pathways were involved in the OTC group,of which 2 most relevant metabolic pathways were linoleic acid and glycerophospholipid metabolism;17 metabolic pathways were involved in the AG group,of which 5 most relevant metabolic pathways were glycerophospholipid metabolism,sphingolipid metabolism,cysteine and methionine metabolism,unsaturated fatty acid biosynthesis and glycine,serine and threonine metabolic pathways.It can be concluded that the mechanism of action of OTC and AG affecting chicken metabolism is very similar,both can affect the normal metabolic function of chickens through changing phospholipid metabolism,fatty acid metabolism and amino acid metabolism,especially the distribution pattern of OTC group is significantly different from the control group.