不同温度下东海原甲藻的细胞死亡

Cell Death of Prorocentrum Donghaiense at Different Temperatures

东海原甲藻赤潮是我国最常见的赤潮,已经给我国海洋生态带来了严重后果,研究其赤潮消亡过程非常重要。本文主要利用流式细胞技术来测定东海原甲藻在不同温度下(16、20、24℃)的细胞生长、死亡率及与死亡相关蛋白caspases-like的活性。结果表明:20℃下东海原甲藻细胞总数和存活细胞数量最多,但死亡率和其他2个温度没有显著差异,且有caspases-like活性的细胞比例和16℃组也无明显差异。可见,东海原甲藻caspases-like蛋白在低温条件下不全是执行PCD的功能,可能有辅助东海原甲藻适应低温的功能。

The bloom of Prorocentrum donghaiense is the most common red tide in China,which has brought serious consequences to marine ecology,therefore,it is very important to study its algal bloom extinction process.In this paper,flow cytometry was mainly used to determine the cell growth,death rate and the caspases-like activity of P.donghaiense at different temperatures(16℃,20℃,24℃).The results showed that,the number of total cells and surviving cells of P.donghaiense were all highest at 20℃,but there was no significant difference in death rate compared to the other two temperatures.And there was no significant difference in the caspases-like activity at 20℃and 16℃.It concluded that the caspases-like protein of P.donghaiense did not all perform the functions of PCD under low temperature,and might have the function of assisting P.donghaiense to adapt to low temperature.