摘要
试验利用生物信息学方法对犬CPV-NS1基因的开放阅读框、理化性质、亲水性/疏水性、蛋白质二级结构、磷酸化和糖基化位点等进行分析。结果表明,犬CPV-NS1基因编码668个氨基酸,等电点5.77,不稳定指数41.44,为不稳定蛋白;亲疏水平均值-0.540,属于亲水蛋白;二级结构依次为无规则卷曲含286个、延伸链204个、含有α螺旋178个,犬CPV-NS1基因60个潜在的磷酸化位点,4个N-糖基化位点。
The open reading frame,physical and chemical properties,hydrophilicity/hydrophobicity,protein secondary structure,phosphorylation and glycosylation sites of canine CPV-NS1 gene were analyzed by bioinformatics methods.The results showed that CPV-NS1 gene encoded 668 amino acids,isoelectric point 5.77,instability index 41.44,which was an unstable protein.The mean value of affinity and sparse level is-0.540,which belongs to hydrophilic protein.The secondary structure consisted of 286 random coils,204 extended chains,178αspirals,60 potential phosphorylation sites and 4 n-glycosylation sites in canine CPV-NS1 gene.
作者
陈生贤
贾明亮
张金海
杜海波
漆亮
CHEN Shengxian;JIA Mingliang;ZHANG Jinhai;DU Haibo;QI Liang(Affair center of animal Husbandry and aquaculture in Jishou,Jishou Hu'nan 416000,China;Sangzhi County Animal health supervision Institute,Zhangjiajie Hu'nan 427199,China;Alagxa League Comprehensive Administrative Law Enforcement Bureau of Luanjingtan Economic and Technological Development Zone,Alxa Inner Mongolia 750312,China;Changsha Animal and Plant Disease Prevention and Control Center,Changsha Hu'nan 410000,China)
出处
《现代畜牧科技》
2023年第7期43-46,共4页
Modern Animal Husbandry Science & Technology
基金
湘西州重点研发计划(2021NC2002)。
