WT 1在不同类型骨髓增殖性疾病病人中的表达及临床意义

Expression and clinical significance of WT 1 in patients with different types of myeloproliferative neoplasm

目的:研究不同亚型的骨髓增殖性肿瘤(MPN)病人WT 1表达水平差异,总结WT 1在MPN中的表达特征,尤其是WT 1基因的相对表达量与慢性粒细胞白血病(CML)病情发展的相关性。方法:运用荧光定量PCR(RQ-PCR)检测56例MPN病人的基因表达类型,以及P210基因、WT 1基因的相对表达量,分析WT 1在不同基因类型MPN中的表达特点。结果:CML组WT 1阳性率与非CML组比较差异有统计学意义(P<0.01),CML组的WT 1阳性病例中WT 1数值与非CML组WT 1阳性病例比较差异亦有统计学意义(P<0.01)。P210+组的WT 1阳性率高于非P210+组(P<0.01),P210+组WT 1阳性病例的WT 1数值与非P210+组WT 1阳性病例比较亦差异有统计学意义(P<0.01)。WT 1和P210基因在CML疾病进程的不同阶段表达差异均有统计学意义(P<0.01),WT 1的数值随着CML进入加速期而明显升高,部分缓解时WT 1的数值则明显下降。结论:MPN病人中WT 1的基因表达具有一定的差异性,且其中P210+的CML病人WT 1相对表达量可以与P210基因的相对表达量一起作为判断CML进展的指标。

Objective:To explore the differential expression levels of WT 1 in patients with different subtypes of myeloproliferative neoplasm(MPN),and to summarize the expression characteristics of WT 1 in MPN,especially the correlation between the relative expression level of the WT 1 gene and the progression of chronic myeloid leukemia(CML).Methods:Fluorescence quantitative PCR(RQ-PCR)was used to detect the gene expression types of 56 MPN patients,as well as the relative expression levels of the P210 gene and WT 1 gene.The expression characteristics of WT 1 in MPN of different gene types were analyzed.Results:There was a statistically significant difference in the WT 1 positive rate between the CML group and the non-CML group(P<0.01),and there was also a statistically significant difference in the WT 1 value between the CML group and the non-CML group(P<0.01).The positive rate of WT 1 in the P210+group was higher than that in the non-P210+group(P<0.01),and the WT 1 value of WT 1 positive cases in the P210+group was also significantly different from that in the non-P210+group(P<0.01).The expression differences of WT 1 and P210 genes at different stages of CML disease progression were statistically significant(P<0.01).The value of WT 1 significantly increased as CML entered the acceleration phase,while the value of WT 1 significantly decreased during partial remission.Conclusions:WT 1 gene expression in MPN has some differences,moreover,the WT 1 relative expression level of P210+CML patients can be used as an index to judge the CML progress together with the relative expression level of P210 gene.