多发性骨髓瘤细胞信号淋巴细胞激活分子家族受体表达及其临床价值

The clinical value and expression of signaling lymphocytic activation molecule family receptors on multiple myeloma cells

目的:探讨多发性骨髓瘤(MM)细胞信号淋巴细胞激活分子(signaling lymphocytic activation molecule,SLAM)家族受体表达的临床价值。方法:选取32例MM病人和20例良性血液病病人,采用流式细胞术分析浆细胞SLAM家族受体成员的表达。正常浆细胞和MM细胞中表达无差异的SLAM家族受体成员利用分辨率度量(Rd)分析其区分MM细胞和粒细胞的能力、设门分析MM细胞百分比,在9份MM细胞中于48 h内和48~72 h间的表达频率。结果:与正常浆细胞比较,MM细胞CD150和CD352表达频率降低(P<0.01),CD48、CD84、CD229和CD319表达频率差异无统计学意义(P>0.05);MM细胞CD48、CD229和CD319的平均荧光强度(MFI)高于淋巴细胞和粒系细胞(P<0.01),CD319的MFI变异系数低于CD48和CD229(P<0.01),而Rd高于CD48和CD229(P<0.01);CD45/CD38联合CD48、CD229或CD319设门与参考设门得到的MM细胞比例呈明显正相关关系(r值分别为0.961、0.953和0.981,P<0.05);MM细胞CD48、CD229和CD319在48 h内和48~72 h间表达频率差异无统计学意义(P>0.05),但48~72 h的CD138阳性频率明显降低(P<0.01)。结论:MM细胞CD150和CD352表达降低,CD319可作为一种标记MM细胞的抗原。

Objective:To explore the clinical value and expression of signaling lymphocytic activation molecule(SLAM)family receptors on multiple myeloma(MM)cells.Methods:Thirty-two MM patients and 20 controls with benign hematologic diseases were chosen,the expression of SLAM family receptors on plasma cells were analyzed by flow cytometry.SLAM factors had no differences in normal plasma cells and MM cells utilizing resolution metrid(Rd)to separate MM cells from granulocytes,analyzing the percentage of MM cells by gating,detecting expression frequencies of SLAM family receptors on 9 MM cells within 48 h and 48-72 h.Results:Compared with normal plasma cells,the expression frequencies of CD150 and CD352 were lower(P<0.01).There were no statistical difference in the expression frequencies of CD48,CD84,CD229 and CD352(P>0.05);CD48,CD229 and CD319 mean fluorescence intensity(MFI)on MM cells were higher than granulocytes and lymphocytes(P>0.05),the coefficient of variation of CD319 MFI was lower than CD48 and CD229(P<0.01),while Rd of CD319 were higher than CD48 and CD229(P<0.01);the percentage of MM cells gated by CD45/CD38 combined with CD48,CD229 or CD319 were positively correlated with the reference method(r=0.961,0.953 and 0.981 respective,P<0.05);frequencies of CD48,CD229 and CD319 on MM cells had no statistical significance within 48 h and 48-72 h(P>0.05).However,the positive frequency of CD138 was significantly decreased at 48-72 h(P<0.01).Conclusions:The expressions of CD150 and CD352 on MM cells were reduced,CD319 could be regarded as a marker antigen for MM cell.