摘要
【目的】研究酵母菌菌株0939-5产挥发性抑菌物质的最佳固态发酵基质及培养条件。【方法】以酵母菌菌株0939-5为材料,采用单因素法,优化该菌株的固体营养成分及培养条件。【结果】酵母菌0939-5的最佳固态发酵基质为玉米粉,最佳碳源及浓度为葡萄糖2%,最佳无机盐及浓度为MgSO 40.1%;菌株0939-5固态发酵的最佳条件为接种浓度1×10^(8) CFU/mL、接种量10%、初始pH4、初始含水量为40%。【结论】在基础培养基的基础上,利用玉米粉作为固体发酵基质,分别以添加葡萄糖2%、MgSO 40.1%,培养条件为接种浓度1×10^(8) CFU/mL、接种量10%、初始pH4、初始含水量为40%时,25℃培养5 d为最佳。
【Objective】To determine the optimal solid-state fermentation substrate and culture conditions for the production of volatile antibacterial substances by using the yeast strain 0939-5 as the test material.【Methods】The solid nutrients and culture conditions of the strain were optimized by single factor method.【Results】The optimum solid-state fermentation substrate of yeast strain 0939-5 was corn meal,the optimum carbon source concentration was glucose 2%,and the optimum inorganic salt concentration was 0.1%MgSO_(4).The optimum conditions for solid-state fermentation of yeast strain 0939-5 were inoculation concentration 1×10^(8) CFU/mL,inoculation volume 10%,initial pH4,and initial water content 40%.【Conclusion】On the basis of the basal medium,corn meal was used as a solid fermentation substrate,and 2%glucose and 0.1%MgSO_(4) were added respectively,and the optimumculture conditions were inoculum concentration of 1×10^(8) CFU/mL,the inoculum volume of 10%,the initial pH of 4,and the initial water content 40%at 25℃for 5 days.
作者
李华静
王晓东
LI Huajing;WANG Xiaodong(Key Laboratory for Oasis Agricultural Pest Management and Plant Resource Utilization at Universities Level of Xinjiang Uygur Autonomous Region/College of Agronomy,Shihezi University,Shihezi Xinjiang 832003,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2023年第7期1773-1779,共7页
Xinjiang Agricultural Sciences
基金
公益性行业(农业)科研专项(201303025)。
关键词
固体发酵
酵母菌
挥发性物质
单因素筛选
solid-state fermentation
yeast
volatile substance
single factor screening