酵母菌0939-5产挥发性抑菌物质固态发酵工艺优化

Study on the solid-state fermentation technology of yeast 0939-5

【目的】研究酵母菌菌株0939-5产挥发性抑菌物质的最佳固态发酵基质及培养条件。【方法】以酵母菌菌株0939-5为材料,采用单因素法,优化该菌株的固体营养成分及培养条件。【结果】酵母菌0939-5的最佳固态发酵基质为玉米粉,最佳碳源及浓度为葡萄糖2%,最佳无机盐及浓度为MgSO 40.1%;菌株0939-5固态发酵的最佳条件为接种浓度1×10^(8) CFU/mL、接种量10%、初始pH4、初始含水量为40%。【结论】在基础培养基的基础上,利用玉米粉作为固体发酵基质,分别以添加葡萄糖2%、MgSO 40.1%,培养条件为接种浓度1×10^(8) CFU/mL、接种量10%、初始pH4、初始含水量为40%时,25℃培养5 d为最佳。

【Objective】To determine the optimal solid-state fermentation substrate and culture conditions for the production of volatile antibacterial substances by using the yeast strain 0939-5 as the test material.【Methods】The solid nutrients and culture conditions of the strain were optimized by single factor method.【Results】The optimum solid-state fermentation substrate of yeast strain 0939-5 was corn meal,the optimum carbon source concentration was glucose 2%,and the optimum inorganic salt concentration was 0.1%MgSO_(4).The optimum conditions for solid-state fermentation of yeast strain 0939-5 were inoculation concentration 1×10^(8) CFU/mL,inoculation volume 10%,initial pH4,and initial water content 40%.【Conclusion】On the basis of the basal medium,corn meal was used as a solid fermentation substrate,and 2%glucose and 0.1%MgSO_(4) were added respectively,and the optimumculture conditions were inoculum concentration of 1×10^(8) CFU/mL,the inoculum volume of 10%,the initial pH of 4,and the initial water content 40%at 25℃for 5 days.