囊胚玻璃化冷冻预平衡时间对辅助生殖妊娠结局的影响

Effect of vitrification pre-balance time of blastula on pregnancy outcome in assisted reproduction

目的探讨囊胚激光皱缩后缩短玻璃化冷冻预平衡时间对临床妊娠及新生儿结局的影响。方法回顾性分析2018年1月至2021年12月于海口玛丽医院进行囊胚复苏移植患者的临床资料,从中筛选501例患者518个移植周期。根据实验室囊胚玻璃化冷冻技术标准操作程序修订前后的预平衡时间将其分为A组(9~10min)和B组(7~8min),比较两组间的妊娠结局和新生儿结局。结果两组患者的年龄、移植胚胎数、卵泡刺激素、体质量指数、不孕年限、原发不孕比例、移植日内膜厚度和内膜方案比较,差异均无统计学意义(P>0.05);两组的囊胚存活率、着床率、临床妊娠率、活产率、早期流产率、多胎妊娠率、母体并发症比较,差异均无统计学意义(P>0.05)。两组的新生儿性别比、孕周、早产儿比例、出生体质量、低出生体重儿、巨大儿、剖宫产、出生缺陷比较,差异均无统计学意义(P>0.05)。结论囊胚激光皱缩后,缩短玻璃化冷冻预平衡时间不影响囊胚复苏移植的妊娠结局。

Objective To investigate the effect of shortening vitrification pre-balance time after laser blastocyst shrinkage on clinical pregnancy and neonatal outcomes.Methods The clinical data of blastocyst resuscitation and transplantation in Haikou Mary Hospital from January 2018 to December 2021 were retrospectively analyzed,and 501 patients for a total of 518 transplantation cycles were screened.The blastocysts were divided into two groups according to the pre-balance time before and after the revision of the standard operating procedure of laboratory vitrification freezing technology:group A(9-10 min)or group B(7-8 min).The pregnancy outcome and neonatal outcomes were compared between the two groups.Results There were no significant differences in age,number of transplanted embryos,follicle-stimulating hormone,body mass index,infertility years,proportion of primary infertility,intima thickness and intima regimen between the two groups(P>0.05).There were no significant differences in blastocyst survival rate,implantation rate,clinical pregnancy rate,live birth rate,early abortion rate,multiple pregnancy rate and maternal complications between the two groups(P>0.05).There were no significant differences in neonatal sex ratio,gestational age,preterm birth rate,birth weight,low birth weight,macrosomia,cesarean section and birth defects between the two groups(P>0.05).Conclusion Shortening the vitrification pre-balance time after laser blastocyst shrinkage did not affect the pregnancy outcome of blastocyst resuscitation transplantation.