Hsa_circRNA_063981作为2型糖尿病诊断标志物的初步研究

Preliminary study of Hsa_circRNA_063981 as a diagnostic marker for type 2 diabetes mellitus

目的分析Hsa_circRNA_063981在2型糖尿病(type 2 diabetes mellitus,T2DM)患者中的表达情况,了解其与临床指标和炎症因子的相关性。方法选取2021年5月至10月于浙江省诸暨市人民医院就诊的40例T2DM患者纳入T2DM组,选取同期40名健康体检者纳入对照组。比较两组受试者的一般临床资料、Hsa_circRNA_063981和白细胞介素-6(interleukin-6,IL-6)的表达水平,Pearson相关性分析Hsa_circRNA_063981与临床指标和炎症指标的相关性,绘制受试者操作特征曲线评估Hsa_circRNA_063981诊断T2DM的潜在价值。结果T2DM组患者的体质量指数、收缩压、舒张压、空腹血糖、糖化血红蛋白、空腹C肽均显著高于对照组,空腹胰岛素显著低于对照组(P<0.05)。T2DM组患者的Hsa_circRNA_063981、IL-6表达均显著高于对照组(P<0.05)。Hsa_circRNA_063981与空腹胰岛素水平呈正相关(r=0.397,P=0.011),与IL-6无相关性。Hsa_circRNA_063981诊断T2DM患者的曲线下面积为0.610,敏感度和特异性分别为33.3%和87.23%。结论Hsa_circRNA_063981对T2DM具有一定的诊断价值。

Objective To investigate the expression of Hsa_circRNA_063981 in patients with type 2 diabetes mellitus(T2DM)and its correlation with clinical indicators and inflammatory factors.Methods A total of 40 T2DM patients treated in Zhuji People’s Hospital of Zhejiang Province from May to October 2021 were included in T2DM group,and 40 healthy physical examination subjects were included in control group during the same period.The general clinical data,expression levels of Hsa_circRNA_063981 and interleukin-6(IL-6)were compared between the two groups,and the correlation between Hsa_circRNA_063981 and clinical and inflammatory indicators was analyzed by Pearson correlation analysis.The potential value of Hsa_circRNA_063981 in the diagnosis of T2DM was evaluated by drawing receiver operating characteristic curve.Results The body mass index,systolic blood pressure,diastolic blood pressure,fasting blood glucose,glycosylated hemoglobin and fasting C-peptide in T2DM group were significantly higher than those in control group,and fasting insulin was significantly lower than that in control group(P<0.05).The expressions of Hsa_circRNA_063981 and IL-6 in T2DM group were significantly higher than those in control group(P<0.05).Hsa_circRNA_063981 was positively correlated with fasting insulin level(r=0.397,P=0.011),but not with IL-6.The area under the curve of Hsa_circRNA_063981 for T2DM diagnosis was 0.610,and the sensitivity and specificity were 33.3%and 87.23%,respectively.Conclusion Hsa_circRNA_063981 is valuable in the diagnosis of T2DM.