普济消毒饮抑制慢性化脓性中耳炎炎症反应

Pujixiaodu decoction inhibits inflammatory reaction of chronic suppurative otitis media

目的探讨普济消毒饮抑制慢性化脓性中耳炎炎症反应的作用。方法采用经鼓膜穿刺途径向鼓室注射铜绿假单胞菌的方法构建慢性化脓性中耳炎小鼠模型。建模成功后,左氧氟沙星组小鼠给予1mg/(kg·d)左氧氟沙星灌胃,普济消毒饮低、中、高剂量组小鼠分别给予2.9g/(kg·d)、5.8g/(kg·d)、11.6g/(kg·d)普济消毒饮浓缩液灌胃,模型组和对照组小鼠均予以等量生理盐水灌胃,每日1次,持续14d。结果左氧氟沙星和普济消毒饮低、中、高剂量均可减轻中耳黏膜组织病变;与模型组比较,左氧氟沙星组和普济消毒饮低、中、高剂量组小鼠的血清肿瘤坏死因子-α、白细胞介素(interleukin,IL)-6、IL-1β、γ干扰素水平降低(P<0.05),Toll样受体4(Toll-like receptor 4,TLR4)、髓样分化蛋白88(myeloid differentiation protein 88,MYD88)、核因子-κB p65(nuclear factor-κB p65,NF-κB p65)基因表达和蛋白水平及磷酸化NF-κB p65降低(P<0.05),上述指标均呈剂量依赖性。结论普济消毒饮可抑制慢性化脓性中耳炎炎症反应,可能与抑制TLR4/MYD88/NF-κB信号通路相关。

Objective To investigate the effect of Pujixiaodu decoction on inhibiting inflammatory reaction of chronic suppurative otitis media.Methods Models of chronic suppurative otitis media were established by injecting Pseudomonas aeruginosa into the tympanic cavity via tympanic membrane puncture.After successful modeling,levofloxacin group was given 1mg/(kg·d)levofloxacin by gavage,the low,medium and high dose Pujixiaodu decoction groups were given 2.9g/(kg·d),5.8g/(kg·d)and 11.6g/(kg·d)concentrated Pujixiaodu decoction by gavage,respectively,model group and control group were given the same amount of normal saline by gavage,once a day for 14 days.Results Levofloxacin and low,medium and high dose Pujixiaodu decoction can reduce the pathological changes of middle ear mucosa.Compared with model group,serum tumor necrosis factor-α,interleukin(IL)-6,IL-1βand interferon-γlevels of mice in levofloxacin group and low,medium and high dose Pujixiaodu decoction groups decreased(P<0.05),Toll-like receptor 4(TLR4),myeloid differentiation protein 88(MYD88),nuclear factor-κB p65(NF-κB p65)gene expression and protein level and phosphorylation NF-κB p65 decreased(P<0.05).The above indexes were dose dependent.Conclusion Pujixiaodu decoction can inhibit the inflammatory response of chronic suppurative otitis media,which may be related to inhibition TLR4/MYD88/NF-κB signaling pathway.