银杏叶提取物金纳多对敌草快诱导大鼠急性肾损伤的改善作用

Ameliorative effect of Ginkgo biloba extract Ginaton on diquat-induced acute kidney injury in rats

目的观察银杏叶提取物金纳多(EGB761)对敌草快(DQ)诱导的大鼠急性肾损伤(AKI)的改善作用,并探讨其作用机制。方法将30只SPF级健康雄性Wistar大鼠分随机为DQ中毒组(DQ组)、敌草快+银杏叶提取物治疗组(DQ+EGB761组)及对照组(NS组),每组各10只。DQ组和DQ+EGB761组予154 mg/kg DQ灌胃(设为第1天),对照组予等剂量生理盐水灌胃。灌胃半小时后DQ+EGB761组予10 mL/kg EGB761腹腔注射(1次/天,连续3次),DQ组、NS组予腹腔注射等剂量生理盐水(1次/天,连续3次)。第4天时麻醉各组大鼠取肾组织,HE染色后观察各组大鼠肾组织病理变化,分别采用实时荧光定量PCR法、WESTERNBlotting法检测大鼠肾组织肾损伤指标肾损伤分子-1(KIM-1)mRNA及蛋白。采用PCR法检测各组大鼠肾组织视黄醇结合蛋白4(RBP4)、核转录因子-κB(NF-κB)及其下游因子肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)mRNA,采用WESTERN Blotting法检测各组大鼠肾组织KIM-1、RBP4、NF-κB蛋白。结果与NS组比较,DQ组大鼠肾小管上皮细胞肿胀,管腔变窄,部分肾小管上皮细胞空泡变性、坏死,炎细胞浸润;与DQ组比较,DQ+EGB761组肾小管上皮细胞肿胀程度及炎症程度较轻。与NS组比较,DQ组大鼠肾组织KIM-1 mRNA、RBP4 mRNA、NF-κB mRNA、TNF-αmRNA、IL-1βmRNA相对表达量高(P均<0.05);与DQ组比较,DQ+EGB761组大鼠肾组织KIM-1 mRNA、RBP4 mRNA、NF-κB mRNA、TNF-αmRNA、IL-1βmRNA相对表达量低(P均<0.05)。与NS组比较,DQ组大鼠肾组织KIM-1、RBP4、NF-κB蛋白相对表达量高(P均<0.05),与DQ组比较,DQ+EGB761组大鼠肾组织KIM-1、RBP4、NF-κB蛋白相对表达量低(P均<0.05)。结论EGB761可减轻DQ诱导的大鼠急性肾损伤,其机制可能与EGB761可以抑制RBP4、NF-κB mRNA及蛋白表达有关。

Objective To observe the ameliorative effect of Ginkgo biloba extract Ginaton(EGB761)on acute kid⁃ney injury(AKI)induced by diquat(DQ)in rats,and to explore its mechanism.Methods Thirty SPF grade healthy male Wistar rats were randomly divided into DQ poisoning group(DQ group),diquat+Ginkgo biloba extract treatment group(DQ+EGB761 group),and control group(NS group),with 10 rats in each group.The rats in the DQ and DQ+EGB761groups were given 154 mg/kg DQ by gavage(day 1).The rats in the control group were given the same dose of normal saline by gavage.Half an hour after gavage,the DQ+EGB761 group was given 10 mL/kg EGB761 intraperitoneal injection(1 time/day,3 times in a row),and the DQ group and the NS group were given the same dose of normal saline in⁃traperitoneally(1 time/day,3 times in a row).The rats were anesthetized on fourth day and kidney tissues were taken.HE staining was used to observed the pathological changes of kidney tissues in each group,and real-time fluorescent quan⁃titative PCR was used to detect the mRNA expression levels of kidney injury molecule-1(KIM-1),retinol-binding protein 4(RBP4),nuclear transcription factor-κB(NF-κB),tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β)in kidney tissues of three groups.Western blotting was used to detect the protein expression levels of KIM-1,RBP4,and NF-κB in the kidney tissues of rats in each group.Results Compared with the NS group,the renal tubular epithelial cells of rats in the DQ group were swollen,the lumen was narrowed,some tubular epithelial cells had vacuolar degeneration and necrosis,and inflammatory cells were infiltrated.Compared with the DQ group,the degree of swelling and inflammation of renal tubular epithelial cells in the DQ+EGB761 group were significantly reduced.Compared with the NS group,the rela⁃tive expression levels of KIM-1 mRNA,RBP4 mRNA,NF-κB mRNA,TNF-αmRNA and IL-1βmRNA in the kidney tis⁃sues of rats in the DQ group increased(all P<0.05).Compared with the DQ group,the relative expression levels of KIM-1 mRNA,RBP4 mRNA,NF-κB mRNA,TNF-αmRNA,and IL-1βmRNA in the kidney tissues of rats in the DQ+EGB761 group were lower(all P<0.05).Compared with the NS group,the relative expression levels of KIM-1,RBP4 and NF-κB proteins in the kidney tissues of rats in the DQ group increased(all P<0.05).Compared with the DQ group,the relative expression levels of KIM-1,RBP4,and NF-κB proteins in the kidney tissues of rats in the DQ+EGB761 group decreased(all P<0.05).Conclusion EGB761 can alleviate acute renal injury caused by DQ,and the mechanism may be that EGB761 can inhibit RBP4,NF-κB mRNA and protein expression.