云南红河地区彝族20个常染色体STR基因座的遗传多态性研究

Study of the Genetic Polymorphism of 20 Autosomal STR Loci in Yi Population in Honghe Region of Yunnan Province

目的调查云南红河地区彝族人群20个常染色体短串联重复序列基因座的遗传多态性,分析云南红河彝族与其他不同群体的遗传关系,为法医学鉴定及群体遗传学研究提供基础数据。方法收集2342份云南红河彝族人群无关个体样本,Chelex-100法提取样本DNA,采用PowerPlex®21 System试剂盒进行PCR复合扩增,用ABI 3130XL自动遗传分析仪对PCR复合扩增产物进行毛细管电泳分离,用GeneMapper ID-X 1.5软件进行STR分型。使用Modified-Powerstats软件统计等位基因频率及匹配概率等法医学参数,并进行Hardy-Weinberg平衡检验。使用Arlequin软件计算F_(st)和P值。使用Phylip软件计算Nei’s遗传距离,使用SPSS软件进行多维尺度分析。应用Mega软件构建相邻连接系统发育树。结果20个STR基因座中共观察到318个等位基因和1314种基因型,各基因座等位基因频率和基因型频率均符合Hardy-Weinberg平衡(P>0.05/20=0.0025)。20个STR基因座的累积个人识别能力达到0.999999999999999999999999729502,累积非父排除概率达到0.999999994995179。F_(st)值、Nei’s遗传距离以及邻接法系统发育树的结果均提示,云南红河彝族与广西彝族、云南越南人群和云南佤族的遗传距离相对较远。结论上述20个STR基因座在云南红河地区彝族群体中具有高度多态性,可用于法医学鉴定和群体遗传学研究。

Objective To investigate the genetic polymorphism of 20 autosome STR loci in the Yi population in Honghe,Yunnan,and analyze the genetic relationship between the Yi people in Honghe,Yunnan and other populations,so as to provide basic data for forensic identification and population genetics research.Method 2342 unrelated healthy Yi individuals in Honghe region of Yunnan Province were investigated.The Chelex-100 method was used to extract DNA.The PowerPlex®21 System reagent kit was used for PCR multiplex amplification.The PCR multiplex amplification products were separated by capillary electrophoresis using ABI 3130XL automatic genetic analyzer,and STR typing was performed using GeneMapper ID-X 1.5 software.The Modified-Powerstats software was used to count the forensic parameters such as allele frequency and matching probability,and the Hardy Weinberg balance test was performed.Using Arlequin software to calculate F_(st)and P values.Phylip software was used to calculate Nei’s Genetic distance,and SPSS software was used for multidimensional scaling analysis.Using Mega software to construct phylogenetic tree.Results 318 alleles and 1314 genotypes were observed for all the 20 loci.All of the loci were in Hardy-Weinberg equilibrium(P>0.05/20=0.0025).The values for the cumulative discrimination power(CDP)and the cumulative probability of exclusion(CPE)of all the 20 STR loci were 0.999999999999999999999999729502 and 0.999999994995179,respectively.The F_(st)values,Nei’s Genetic distance and the results of the phylogenetic tree all suggested that the genetic distances between Yunnan Honghe Yi population and Guangxi Yi population,Yunnan Vietnam population and Yunnan Va population were relatively far.Conclusion The results demonstrated that these 20 STR loci were highly polymorphic and informative in Honghe Yi population and could be applied in forensic identification and population genetics research.