高效液相法同时测定市售牡丹皮配方颗粒中四种活性成分的含量

Simultaneous determination of four active ingredients in commercial Moutan Cortex formula granules by high performance liquid chromatography

目的建立同时测定牡丹皮配方颗粒中4种活性成分(没食子酸、儿茶素、芍药苷、丹皮酚)含量的方法,并对山东省内市售的22批牡丹皮配方颗粒的4种活性成分的含量进行测定,分析不同提取工艺对活性成分含量的影响。方法采用Agilent ZORBAX SB-C18柱,以乙腈-0.2%磷酸溶液为流动相,梯度洗脱,流速1.0 ml/min,检测波长230 nm,柱温:35℃,进样量:10μl。结果没食子酸、儿茶素、芍药苷、丹皮酚分别在1.07~107.34μg/ml(R^(2)=0.999)、1.08~108.12μg/ml(R^(2)=0.998)、1.01~101.14μg/ml(R^(2)=0.999)、1.04~104.24μg/ml(R^(2)=0.999)范围内与峰面积呈良好的线性关系;平均回收率分别为95.26%、94.66%、95.54%,95.57%,RSD分别为0.2%、0.5%、0.2%、0.3%。测定市售的22批样品,没食子酸、儿茶素、芍药苷和丹皮酚的含量分别为6.028~21.996 mg/g、0.032~0.209 mg/g、11.147~26.269 mg/g、0.215~11.499 mg/g。结论该含量测定方法快速、简单,可为规范和控制牡丹皮配方颗粒质量提供科学方法和依据。

Objective To establish a method for simultaneous determination of the content of four active ingredients(gallic acid,catechin,paeoniflorin,paeonol)in Moutan Cortex formula granules,and to determine the content of the four active ingredients in 22 batches of Moutan Cortex formula granules sold in Shandong Province,and to analyze the influence of different extraction processes on the content of active ingredients.Methods Agilent ZORBAX SB-C18 column was used,with acetonitrin-0.2%phosphoric acid solution as the mobile phase,gradient elution,flow rate 1.0 ml/min,detection wave length 230 nm,column temperature:35℃,injection volume:10μl.Results There were good linear relationships of gallic acid,catechin,paeoniflorin and paeonol to peak area in the range of 1.07-107.34μg/ml(R^(2)=0.999),1.08-108.12μg/ml(R^(2)=0.998),1.01-101.14μg/ml(R^(2)=0.999)and 1.04-104.24μg/ml(R^(2)=0.999),respectively.The average recovery rates were 95.26%,94.66%,95.54%,95.57%,and the RSD were 0.2%,0.5%,0.2%,0.3%,respectively.In 22 batches of commercial samples,the concentrations of gallic acid,catechin,paeoniflorin and paeonol were 6.028-21.996 mg/g,0.032-0.209 mg/g,11.147-26.269 mg/g and 0.215-11.499 mg/g,respectively.Conclusion The content determination method is fast and simple,which can provide a scientific method and basis for standardizing and controlling the quality of Moutan Cortex formula granules.