姜黄素联合单胺氧化酶A抑制剂氯吉灵对肺癌细胞增殖的影响

Effect of curcumin combined with monoamine oxidase inhibitor clorgyline on lung cancer cell proliferation

目的 研究姜黄素(curcumin, Cur)联合单胺氧化酶A(monoamine oxidase A,MAOA)抑制剂氯吉灵(clorgyline, Clo)对肺癌细胞增殖的影响及作用机制。方法 CCK-8法检测不同浓度Cur和Clo对肺癌细胞H460增殖的作用;Western Blot检测Cur、Clo、Cur联合Clo对H460细胞MAOA表达水平的影响;裸鼠移植瘤模型研究Cur、Clo、Cur联合Clo对H460细胞体内增殖的抑制作用;免疫组织化学(immunohistochemistry, IHC)检测裸鼠移植瘤组织中各治疗组的Ki67蛋白表达变化,TUNEL染色检测裸鼠移植瘤组织中各治疗组细胞的凋亡水平。结果 Cur、 Clo、Cur联合Clo对H460细胞增殖均有抑制作用,且呈现剂量依赖性;并且能够抑制H460细胞裸鼠移植瘤的生长(P<0.01),降低Ki67的表达水平,增加细胞凋亡,且联合组呈现更显著的抑制效果(P<0.01)。结论Cur联合Clo抑制H460肺癌细胞的增殖,其机制可能与降低细胞增殖蛋白Ki67的表达、增加细胞凋亡有关。

Objective To study the effect of curcumin(Cur)combined with monoamine oxidase A(MAOA)inhibitor clorgyline(Clo)on lung cancer cell proliferation and its mechanism.Methods The effect of various concentrations of Cur and Clo on proliferation of the lung cancer cell line H460 was assessed by a CCK-8 assay.Western Blot was used to analyze the effect of Cur,Clo,and Cur combined with Clo on MAOA expression in H460 cells.The inhibitory effect of Cur,Clo,and Cur combined with Clo on H460 cell proliferation was further examined in a xenograft model in nude mice.Immunohistochemistry was used to assess the effect of Clo on Ki67 protein expression in tumor tissue of the xenograft model.TUNEL staining was used to detect apoptosis in various treatment groups of xenografted tumor tissue.Results Cur,Clo,and Cur combined with Clo inhibited H460 cell proliferation in a dose-dependent manner and H460 cell growth in the xenograft model(P<0.01),decreased the Ki67 expression,and increased apoptosis.Importantly,the combined treatment group showed more significant inhibitory effects(P<0.01).Conclusions Cur combined with Clo inhibits the proliferation of H460 lung cancer cells,which may be related to reducing expression of the cell proliferation protein Ki67 and increasing apoptosis.