不同浓度赤霉素对油樟种子生理及萌芽的影响

Effects of Gibberellin with Different Concentrations on Physiological and Germination of Cinnamomum longepaniculatum Seeds

油樟(Cinnamomum longepaniculatum(Gamble)N.Chao ex H.W.Li)是国家Ⅱ级重点保护植物,也是我国特有、盛产天然芳香油的经济林木,而目前油樟种子研究较少,通过赤霉素处理后油樟种子萌芽率及其生理指标如何变化目前还未有深入研究。通过赤霉素(Gibberellins,GAs)处理油樟种子以期促进油樟种子快速萌发,通过各项生理指标的测定与形态结构的观察,进一步丰富油樟种子萌芽的理论基础。以油樟种子为实验材料,采用不同浓度赤霉素(0.2、0.4、0.6、1 g·L^(−1))和不同时间(24 h、48 h)处理,对比不同处理生理指标以及种胚发育情况,0.4 g·L^(−1)的赤霉素处理可显著提升油樟种子SOD、CAT活性,显著降低油樟种子MDA含量,显著提高可溶性蛋白质含量,胚的形态发育明显优于其他处理,胚乳细胞连接密度较小、脂滴含量较多、细胞壁较薄。因此0.4 g·L^(−1)赤霉素浸种48 h有利于促进油樟种子萌发。

Cinnamomum longepaniculatum is a second-class key protected plant in China,and it is also an economic forest rich in natural aromatic oil,which is unique to China.Currently,there were few studies on Cinnamomum longepaniculatum seeds,and there was no in-depth study on how the germination rate and physiological index of Cinnamomum longepaniculatum seeds change after gibberellin treatment.In order to promote the rapid germination of Cinnamomum longepaniculatum seeds,the plant growth regulator GAs was used to treat them.Through the determination of various physiological index and the observation of morphological structure,the theoretical basis of Cinnamomum longepaniculatum seeds germination could be further enriched.Taking Cinnamomum longepaniculatum seeds as the experimental materials,four concentrations of GAs(0.2 g·L^(−1),0.4 g·L^(−1),0.6 g·L^(−1),1 g·L^(−1))treat the seeds for 24 h and 48 h,the physiological indexes and embryo development were compared.The results showed that 0.4 g·L^(−1)gibberellin treatment could significantly increase SOD and CAT activities,significantly decreased MDA content and significantly increased soluble protein content in Cinnamomum longepaniculatum seeds.The morphological development of embryos was obviously better than other treatments,and the endosperm cell connection density was smaller,the lipid droplets content was higher and the cell wall was thinner.Therefore,soaking seeds with 0.4 g·L^(−1)GAs for 48 h was beneficial to promote the germination of Cinnamomum longepaniculatum seeds.