基于UPLC指纹图谱的穿心莲不同部位化学成分差异研究

Study on chemical constituents of different parts of Andrographis paniculata based on UPLC fingerprint

目的建立UPLC指纹图谱比较穿心莲主茎、侧茎、叶3个部位化学成分的差异性,为穿心莲药材质量评价提供依据。方法采用CORTECS UPLC T3色谱柱(100 mm×2.1 mm,1.6μm);以乙腈-0.1%磷酸溶液为流动相进行梯度洗脱。运用相似度评价、热图聚类分析、主成分分析、正交偏最小二乘法判别分析对14批(S1~S14)穿心莲主茎、侧茎、叶的UPLC指纹图谱进行研究。结果建立的UPLC指纹图谱中,穿心莲主茎、侧茎、叶均确定了13个共有峰,并指认出木犀草素-7-O-β-D葡萄糖醛酸苷、穿心莲内酯、新穿心莲内酯、14-去氧穿心莲内酯和脱水穿心莲内酯5个特征峰;除S3、S7、S9外,穿心莲主茎、侧茎、叶UPLC指纹图谱与相应对照指纹图谱的相似度均>0.900;热图聚类分析和主成分分析结果均可区分主茎、侧茎和叶;正交偏最小二乘法判别分析筛选出了4个VIP值>1、P<0.05的差异性成分。结论本研究较为整体、全面、真实地反映了穿心莲不同部位化学成分的差异性,对穿心莲药材的质量控制及评价具有重要意义。

Objective To establish UPLC fingerprint to compare the differences of chemical constituents in the main stem,lateral stem,and leaf of Andrographis paniculata,so as to provide a basis for the quality evaluation of Andrographis paniculata.Methods CORTECS UPLC T3 column(100 mm×2.1 mm,1.6μm)was used;gradient elution was performed with acetonitrile-0.1%phosphoric acid solution as mobile phase.The UPLC fingerprints of 14 batches(S1-S14)of main stem,lateral stem,and leaf of Andrographis paniculata were studied by similarity evaluation,heat map cluster analysis,principal component analysis,and orthogonal partial least squares discriminant analysis.Results In the established UPLC fingerprint,13 common peaks were identified in the main stem,lateral stem,and leaf of Andrographis paniculata,and identified five characteristic peaks of luteolin-7-O-β-D glucuronide,andrographolide,neoandrographolide,14-deoxyandrographolide,and anhydroandrographolide;except for S3,S7,and S9,the similarity between the UPLC fingerprints of main stem,lateral stem,and leaf of Andrographis paniculata and the corresponding control fingerprints was greater than 0.900;the results of heat map cluster analysis and principal component analysis could distinguish the main stem,lateral stem,and leaves;the orthogonal partial least squares discriminant analysis screened out four different components with VIP value>1,P<0.05.Conclusion This study reflects the differences of chemical constituents in different parts of Andrographis paniculata as a whole,comprehensively and truly,and is of great significance to the quality control and evaluation of Andrographis paniculata.