子宫颈鳞癌中炎症小体AIM2与GSDMD的表达及临床意义

Expressions and clinical significance of AIM2 inflammasome and GSDMD in cervical squamous cell carcinoma

目的探究炎症小体黑色素瘤缺乏因子2(AIM2)、消皮素D(GSDMD)在子宫颈鳞癌组织中的表达及与临床病理特征的关系。方法利用癌症基因组图谱(TCGA)数据库分析炎症小体AIM2、GSDMD在子宫颈鳞癌中的表达情况;选取2019年6月至2021年6月徐州市中心医院手术切除的子宫颈鳞癌患者的癌组织80例及子宫肌瘤患者的正常子宫颈组织45例,采用免疫组化和PCR技术检测两组标本中炎症小体AIM2、GSDMD的表达水平,并分析两者之间相关性及其与临床病理特征的关系。结果(1)TCGA数据库结果发现,与正常宫颈组织比较,在子宫颈鳞癌中炎症小体AIM2表达显著升高(P<0.01),GSDMD轻度增高(P>0.05)。(2)免疫组化结果显示AIM2、GSDMD在子宫颈鳞癌组织中的表达显著高于正常宫颈组织,差异有统计学意义(P<0.05)。(3)PCR结果显示炎症小体AIM2、GSDMD mRNA在子宫颈鳞癌组织中的表达水平显著高于正常宫颈组织,差异有统计学意义(P<0.05)。(4)子宫颈鳞癌组织中炎症小体AIM2表达与分化程度、FIGO分期、淋巴结转移、高危型HPV感染有关联(P<0.05);GSDMD表达与分化程度、FIGO分期、脉管浸润有关联(P<0.05)。结论炎症小体AIM2、GSDMD在子宫颈鳞癌的表达明显高于正常宫颈组织,并与子宫颈鳞癌患者的临床病理特征密切相关。

Objective To explore the expression levels of inflammasome absent in melanoma 2(AIM2)and gasdermin-D(GSDMD)in cervical squamous cell carcinoma and their relationship with clinicopathological features.Methods The expression levels of AIM2 inflammasome and GSDMD in cervical squamous cell carcinoma tissues were observed using the Cancer Genome Atlas(TCGA)database.From June 2019 to June 2021,80 cases of cancer tissue samples of cervical squamous cell carcinoma patients(case group)and 45 cases of normal cervical tissue samples of uterine fibroid(control group)which obtained by surgery in Xuzhou Central Hospital were collected to detect the expression levels of AIM2 inflammasome and GSDMD by immunohistochemistry(IHC)and polymerase chain reaction(PCR).The associations of AIM2 inflammasome and GSDMD levels with the clinicopathological characteristics were analyzed.Results TCGA database revealed that compared with normal cervical tissues,the expression level of AIM2 inflammasome was sinificantly increased(P<0.01),while GSDMD was mildly increased in carcinoma tissues(P>0.05).Both IHC and PCR showed that the expression levels of AIM2 inflammasome and GSDMD in case group were significantly higher than those in control group(P<0.05).In case group,the expression level of AIM2 inflammasome was statistically associated with the degree of cancer cell differentiation,FIGO stage,lymph node metastasis and high-risk HPV infection(P<0.05),and the expression level of GSDMD was related to the degree of cancer cell differentiation,FIGO stage and vascular infiltration(P<0.05).Conclusion The expression levels of AIM2 inflammasome and GSDMD in cervical squamous cell carcinoma tissues are significantly higher than those in normal cervical tissues and are closely related to the clinical and pathological features of cervical squamous cell carcinoma.