基于非线性光学显微镜无标记探测胃粘膜肠上皮化生

Nonlinear optical microscopy for label-freely detecting gastric intestinal metaplasia

胃粘膜肠上皮化生被广泛认为是胃癌的前兆。早期发现和准确诊断将具有重要的临床意义。在本工作中,我们提出采用非线性光学显微镜对胃粘膜肠上皮化生进行无标记探测。成像结果表明,该技术有能力区分正常和病变组织,并能进一步区分肠上皮化生的亚型。此外,我们分别测量正常和异常组织上皮细胞的核面积,以说明胃黏膜从正常到肠上皮化生发展过程中的细胞形态变化。本研究说明非线性光学显微镜具备诊断胃粘膜肠上皮化生的潜力,将为进一步研究这种病变提供一种新方法。

Intestinal metaplasia has been widely considered as a precursor of gastric cancer,and early detection and accurate diagnosis will have important clinical significance.Therefore,nonlinear optical microscopy using two-photon excited fluorescence combined with second harmonic generation was used for investigating gastric mucosal intestinal metaplasia in this work,and imaging results showed that this microscope has the ability to directly identify this lesion from normal tissues in the absence of labels and to further differentiate the subtyping of intestinal metaplasia.Furthermore,nuclear areas from normal gastric mucosa and intestinal metaplasia subtypes were measured respectively to show the cell changes while gastric mucosa developed from normal to intestinal metaplasia.This study may provide an approach for further researching these diseases in the future,and highlights the potential of nonlinear optical microscopy as a diagnostic tool for label-free identification of gastric intestinal metaplasia.