黄绿卷毛菇胞外多糖发酵条件的优化及其生物活性研究

Optimization of Fermentation Conditions and Biological Activities of Exopolysaccharides From Floccularia luteovirens

真菌胞外多糖是具有多种生物学功能的重要天然产物。为探究新的发酵方法以提高黄绿卷毛菇胞外多糖(FLEP)的产量,本试验以黄绿卷毛菇菌株WMY10为供试菌株,通过响应面法优化了微颗粒粒径、超声时间、表面活性剂对FLEP产量的影响,并对其抗氧化活性和吸湿保湿性进行了测定。结果表明,最佳条件为吐温80质量浓度0.4 g·L^(-1)、微颗粒粒径100~200目、超声时间20 min,在此条件下FLEP产量为2.44 g·L^(-1),是对照组的1.5倍。抗氧化试验表明,FLEP具有较好的体外抗氧化活性。对1,1-二苯基-2-三硝基苯肼(DPPH)清除率可达到71.0%,对亚铁离子螯合率达到58.2%,对2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)的清除能力为0.044 mmol·g^(-1)Trolox。以甘油、透明质酸、壳聚糖为对照,在相对湿度为43%和81%的环境下,FLEP的吸湿效果优于壳聚糖;在相对湿度为43%的环境下,FLEP的保湿效果优于透明质酸和壳聚糖;在干燥环境中,FLEP的保湿效果最好。综上,FLEP可以作为天然抗氧化剂或水分保持剂应用于食品、药品和化妆品行业。本研究为FLEP的高效生产和应用提供了理论基础。

Fungal exopolysaccharides are important natural products with diverse biological functionalities.To explore novel fermentation approaches to enhance the production of exopolysaccharides derived from Floccularia luteovirens(FLEP),response surface methodology was employed to optimize the influence of microparticle size,ultrasonic stimulus time,and surfactant concentration on the yield of FLEP,utilizing strain WMY10 as the experimental strain.In addition,the antioxidant properties,as well as the moisture absorption and retention capabilities of FLEP,were evaluated.The results demonstrated that the optimal conditions involved a Tween 80 concentration of 0.4 g·L^(-1),a microparticle size ranging from 100 to 200 mesh,and an ultrasonic stimulus time of 20 minutes.Under these optimized conditions,FLEP exhibited a maximum yield of 2.44 g·L^(-1),which was 1.5 times higher than that of the control group.Furthermore,FLEP displayed considerable in vitro antioxidant activity,with chelation capacities of 71.0%and 58.2%in the DPPH and ferrous ion chelation tests,respectively.The ABTS radical scavenging capacity of FLEP was determined to be 0.044 mmol·g^(−1)Trolox.Moreover,in comparison to glycerol,hyaluronic acid,and chitosan,FLEP demonstrated superior moisture absorption at relative humidities of 43%and 81%.At a relative humidity of 43%,FLEP also exhibited better moisture retention ability than hyaluronic acid and chitosan.FLEP displayed the most effective moisture retention capacity under dry conditions.In summary,FLEP could be used as a natural antioxidant or water retention agent in the food,pharmaceutical,and cosmetic industries.This study provides a theoretical foundation for the efficient production and application of FLEP.