摘要
目的探讨二烯丙基二硫(DADS)通过RORα/β-catenin信号对人胃癌MGC803细胞迁移侵袭与上皮-间质转化(EMT)的影响。方法MGC803细胞实验分为MGC803组、DADS组、SR1078组、SR1078+DADS组。MTT检测细胞增殖能力;细胞划痕和Transwell实验分别检测细胞迁移与侵袭能力。Western blotting与免疫荧光检测RORα/β-catenin信号与EMT相关分子表达情况,免疫共沉淀检测RORα蛋白与β-catenin蛋白结合能力,相差显微镜观察细胞形态改变。结果与MGC803组比较,DADS组、SR1078组细胞增殖能力分别呈时间依赖性降低(P<0.05);细胞迁移与侵袭能力降低(P<0.05);RORα蛋白与E-cadherin明显上调(P<0.05),而核内β-catenin、转化生长因子-β1、Rac1与Vimentin表达下调(P<0.05);RORα与β-catenin结合明显减少(P<0.05);长梭形细胞减少,异型性降低(P<0.05)。SR1078+DADS组的上述指标结果更明显(P<0.05)。结论DADS通过RORα/β-catenin信号抑制人胃癌MGC803细胞增殖、迁移侵袭与EMT,提示DADS与SR1078的作用相似。
Aim To investigate the effect of diallyl disulfide(DADS)on human gastric cancer MGC803 cell migration and invasion and epithelial-mesenchymal transition(EMT)via RORα/β-catenin signaling pathway.Methods The MGC803 cell experiment was divided into MGC803 control group,DADS group,SR1078 group and SR1078+DADS group.MTT was used to detect cell proliferation.Cell migration and invasion ability were detected by cell scratch and Transwell assay,respectively.Western blotting and immunofluorescence were used to detect the expression of RORα/β-catenin signaling pathway and EMT-related molecules.Co-immunoprecipitation was used to detect the binding ability of RORαprotein withβ-catenin protein,and the cell morphology was observed by phase contrast microscopy.Results Compared with the control group,the proliferation capacity of DADS group and SR1078 group was decreased in a time dependent manner(P<0.05).The ability of cell migration and invasion was decreased(P<0.05).RORαprotein and E-cadherin were significantly up-regulated(P<0.05),whereasβ-catenin,transforming growth factor-β1,Rac1 and Vimentin expression were down-regulated(P<0.05).The binding of RORαwithβ-catenin was significantly decreased(P<0.05).The number of long spindle cells decreased and the atypia decreased(P<0.05).The above effects of SR1078+DADS group were more significant(P<0.05).Conclusion DADS inhibits the proliferation,migration,invasion,and EMT of human gastric cancer MGC803 cells through RORα/β-catenin signaling pathway,indicating similar effects of DADS as SR1078.
作者
赵晓红
苏坚
苏波
夏红
刘芳
苏琦
ZHAO Xiaohong;SU Jian;SU Bo;XIA Hong;LIU Fang;SU Qi(Cancer Research Institute,Hengyang Medical School,University of South China,Hunan Key Laboratory of Cancer Cellular and Molecular Pathology,Hengyang 421001,Hunan,China;Department of Obstetrics and Gynecology,Hainan Women and Children's Medical Center,Haikou 570206,Hainan,China;Department of Pathology,Affiliated Second Hospital,Hengyang Medical School,University of South China,Hunan Clinical Research Center for Gastric Cancer Prevention and Treatment,Hengyang 421001,Hunan,China;Institute of Pharmacy and Pharmacology,Hengyang Medical School,University of South China,Key Laboratory for Pharmacoproteomics of Hunan Provincial University,Hengyang 421001,Hunan,China)
出处
《中南医学科学杂志》
CAS
2023年第4期475-480,共6页
Medical Science Journal of Central South China
基金
国家自然科学基金(81374013,81973532)。
