基于香气活力值的加拿大烟叶主流烟气关键酸性香气成分分析

Analysis of Key Acidic Aroma Components of Canadian Tobacco Leaves Mainstream Smoke Based on Odor Activity Value

【目的】明确加拿大烟叶主流烟气中的关键酸性香气成分,为开发具有加拿大烟叶风格特征的烟用香料提供参考。【方法】以加拿大烟叶主流烟气酸性香气成分为研究对象,通过柱层析和中压制备技术进行合理有效的分割,以感官评价为导向,结合GC-MS分析确定特征组分,对其中的重要酸性香气成分进行定量分析并计算相应的香气活力值(odor activity value,OAV)。【结果】(1)加拿大烟叶烟气酸性特征组分主要是小分子羧酸和芳香族羧酸,确定了其中16种重要酸性香气成分,小分子羧酸组分中异戊酸质量浓度最高(1.09 mg/mL),芳香族羧酸组分中苯乙酸质量浓度最高(6.44 mg/mL);(2)16种酸性香气成分的OAV均大于1,其中异戊酸和苯乙酸的OAV最大。【结论】异戊酸和苯乙酸是加拿大烟叶主流烟气中的关键酸性香气成分。

[Purpose]To identify the key acidic aroma components in Canadian tobacco leaves mainstream smoke,providing a reference for the development of flavor with Canadian tobacco style characteristics.[Methods]Using the acidic components of mainstream smoke of Canadian tobacco leaves as the research object,the reasonable and effective segmentation was carried out through column chromatography and medium pressing preparation technology.Guided by sensory evaluation and combined with GC-MS analysis,the characteristic groups were determined.The important acidic aroma components were quantitatively analyzed and the corresponding odor activity value(odor activity value,OAV)was calculated.[Results]1)The acidic characteristic components of Cana-dian tobacco leaves smoke were mainly small molecule acid and aromatic acid groups,and 16 import-ant acidic aroma components of them were identified.The mass concentration of isovaleric acid(1.09 mg/mL)and phenylacetic acid(6.44 mg/mL)were the highest in small molecular acid and aro-matic acid group,respectively.2)The OAV of 16 components were all greater than one,among which isovaleric acid and phenylacetic acid had the largest OAV.[Conclusion]Isovaleric acid and phenylacetic acid are the key acidic aroma components in mainstream smoke of Canadian tobacco leaves.